WARS Kit ELISA

N° du produit ABIN6951745

Détail du produit WARS Kit ELISA

Antigène: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human WARS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: WARS (Tryptophanyl-tRNA Synthetase (WARS))

Autre désignation: WARS (WARS Produits)

Synonymes: GAMMA-2 Kit ELISA, IFI53 Kit ELISA, IFP53 Kit ELISA, zgc:73274 Kit ELISA, wars Kit ELISA, MGC53671 Kit ELISA, WARS Kit ELISA, TrpRS Kit ELISA, WRS Kit ELISA, 85D-WRS Kit ELISA, CG9735 Kit ELISA, Dmel\\CG9735 Kit ELISA, WRS-85D Kit ELISA, l(3)03559 Kit ELISA, l(3)03560 Kit ELISA, l(3)3559 Kit ELISA, gamma-2 Kit ELISA, ifi53 Kit ELISA, ifp53 Kit ELISA, GB14934 Kit ELISA, ECK3371 Kit ELISA, JW3347 Kit ELISA, tryptophanyl-tRNA synthetase Kit ELISA, tryptophanyl-tRNA synthetase S homeolog Kit ELISA, Tryptophanyl-tRNA synthetase Kit ELISA, tryptophanyl-tRNA synthetase L homeolog Kit ELISA, tryptophan--tRNA ligase, cytoplasmic Kit ELISA, WARS Kit ELISA, wars Kit ELISA, wars.S Kit ELISA, Wars Kit ELISA, TrpRS Kit ELISA, wars.L Kit ELISA, LOC727582 Kit ELISA, trpS Kit ELISA, trpS2 Kit ELISA

ID gène: 7453

UniProt: P23381