TXNRD1 Kit ELISA
-
- Antigène Voir toutes TXNRD1 Kits ELISA
- TXNRD1 (Thioredoxin Reductase 1 (TXNRD1))
-
Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Rat TXNRD1 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product TXNRD1 Kit ELISA
-
-
- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Date de péremption
- 6 months
-
- Antigène Voir toutes TXNRD1 Kits ELISA
- TXNRD1 (Thioredoxin Reductase 1 (TXNRD1))
- Autre désignation
- TXNRD1 (TXNRD1 Produits)
- Synonymes
- TrxR1 Kit ELISA, cb682 Kit ELISA, fb83a08 Kit ELISA, wu:fb83a08 Kit ELISA, GB14972 Kit ELISA, MGC85342 Kit ELISA, NADPH-dependent thioredoxin reductase C Kit ELISA, T32G6.20 Kit ELISA, T32G6_20 Kit ELISA, GRIM-12 Kit ELISA, TR Kit ELISA, TR1 Kit ELISA, TRXR1 Kit ELISA, TXNR Kit ELISA, Tr Kit ELISA, thioredoxin reductase 1 Kit ELISA, thioredoxin reductase 1 L homeolog Kit ELISA, NADPH-dependent thioredoxin reductase C Kit ELISA, ThioRedoXin Reductase Kit ELISA, TXNRD1 Kit ELISA, txnrd1 Kit ELISA, Trxr-1 Kit ELISA, txnrd1.L Kit ELISA, NTRC Kit ELISA, trxr-1 Kit ELISA, Txnrd1 Kit ELISA
- UniProt
- O89049
- Pathways
- Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Cell RedoxHomeostasis
-