YWHAB Kit ELISA

N° du produit ABIN6951750

Détail du produit YWHAB Kit ELISA

Antigène: YWHAB (14-3-3 alpha + beta (YWHAB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human YWHAB ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: YWHAB (14-3-3 alpha + beta (YWHAB))

Autre désignation: YWHAB (YWHAB Produits)

Synonymes: GW128 Kit ELISA, HS1 Kit ELISA, KCIP-1 Kit ELISA, YWHAA Kit ELISA, 1300003C17Rik Kit ELISA, wu:fb22g04 Kit ELISA, wu:fr86a12 Kit ELISA, ywhab Kit ELISA, ywhab2 Kit ELISA, zgc:64092 Kit ELISA, 14-3-3beta Kit ELISA, fb80c08 Kit ELISA, fk41f08 Kit ELISA, wu:fb80c08 Kit ELISA, wu:fd11d01 Kit ELISA, wu:fk41f08 Kit ELISA, ywhab1 Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide b Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta S homeolog Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta L homeolog Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide a Kit ELISA, YWHAB Kit ELISA, Ywhab Kit ELISA, ywhabb Kit ELISA, ywhab.S Kit ELISA, ywhab.L Kit ELISA, ywhaba Kit ELISA

ID gène: 7529

UniProt: P31946

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location