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EDAR Kit ELISA

EDAR Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951754
  • Antigène Voir toutes EDAR Kits ELISA
    EDAR (Ectodysplasin A Receptor (EDAR))
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human EDAR ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Voir toutes EDAR Kits ELISA
    EDAR (Ectodysplasin A Receptor (EDAR))
    Autre désignation
    EDAR (EDAR Produits)
    Synonymes
    rs3 Kit ELISA, edar Kit ELISA, EDAR Kit ELISA, MGC88893 Kit ELISA, DL Kit ELISA, ECTD10A Kit ELISA, ECTD10B Kit ELISA, ED1R Kit ELISA, ED3 Kit ELISA, ED5 Kit ELISA, EDA-A1R Kit ELISA, EDA1R Kit ELISA, EDA3 Kit ELISA, HRM1 Kit ELISA, dl Kit ELISA, RGD1561714 Kit ELISA, ectodysplasin A receptor Kit ELISA, ectodysplasin-A receptor Kit ELISA, edar Kit ELISA, EDAR Kit ELISA, Edar Kit ELISA
    ID gène
    10913
    UniProt
    Q9UNE0
    Pathways
    Tube Formation, Ubiquitin Proteasome Pathway
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