Enzyme Linked Immunosorbent Assays (ELISA) for the qualitative detection of the COVID-19 IgM in human serum.
Type d'échantillon
Serum
Analytical Method
Qualitative
Attributs du produit
gM is first immunoglobulin to be produced in response to an antigen and will be primarily detectable during the early onset of the disease. IgM antibodies begin to show positive after 3-5 days of onset.
Ingrédients
COVID-19 IgM coated Microplate
COVID-19 IgM Sample Diluent
HRP labeled COVID-19 Antigen
ELISA Wash Concentrate
ELISA HRP Substrate
ELISA Stop Solution
COVID-19 IgM Negative Control
COVID-19 IgM Positive Control
Matériel non inclus
Precision single channel pipettes capable of delivering 10 µL, 25 µL, 100 µL, and 1000 µL, etc.
Repeating dispenser suitable for delivering 100 µL.
Disposable pipette tips suitable for above volume dispensing.
Disposable 12 x 75 mm or 13 x 100 glass tubes.
Disposable plastic 1000 mL bottle with caps.
Aluminum foil.
Deionized or distilled water.
Plastic microtiter well cover or polyethylene film.
ELISA multichannel wash bottle or automatic (semi-automatic) washing system.
Spectrophotometric microplate reader capable of reading absorbance at 450 nm.
Indications d'application
National Health Commission of the People's Republic of China states that IgM antibodies begin to show positive after 3-5 days of onsett of COVID-19
Plaque
Pre-coated
Protocole
Assay controls and samples are added to the microtiter wells of a microplate that was coated with a anti-human IgM specific antibody. After the first incubation period, the unbound protein matrix is removed with a subsequent washing step. A horseradish peroxidase (H labeled recombinant COVID-19 antigen is added to each well. After an incubation period, an immunocomplex of "Anti-hIgM antibody COVID-19 IgM antibody - HRP labeled COVID-19 antigen" is formed if there is novel coronavirus IgM antibody present in the tested materials. The unbound tracer antigen is removed by the subsequent washing step. HRP-labeled COVID-19 antigen tracer bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antigen bound to the coronavirus IgM on the wallof the microtiter well is proportional to the amount of the coronavirus IgM antibody level in the tested materials.
Restrictions
For Research Use only
Stock
4 °C
Stockage commentaire
This test kit must be stored at 2 - 8°C upon receipt. For the expirati date of the kit refer to the label on the kit box. All components are stable until this expiration date.
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