IL-6 Kit ELISA

N° du produit ABIN6957175

Détail du produit IL-6 Kit ELISA

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Lapin

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 pg/mL - 200 pg/mL

Seuil minimal de détection: 3.12 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL6 in rabbit serum, plasma, tissue homogenates, cell lysates, cell culture supernates.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6)

Sensibilité: 1.24 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 0.5 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 200pg/mL. Prepare 7 tubes containing 0.25 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 200pg/mL, 100pg/mL, 50pg/mL, 25pg/mL, 12.5pg/mL, 6.25pg/mL, 3.12pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Stock: 4 °C/-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Date de péremption: 6 months

Références pour IL-6 Kit ELISA (ABIN6957175)

Mousa, Allemailem, Alhumaydhi, Alrumaihi, Almatroudi, Aljasir, Alwashmi, Al Rugaie, Soliman, Aljohani, Al Abdulmonem, Ahmed, Khan, Khan, AlSuhaymi, Alsugoor, Al-Megrin, Elsayed: "Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits." dans: , Vol. 2022, pp. 2863023, (2022) (PubMed).

Mikolka, Kopincova, Kosutova, Kolomaznik, Calkovska, Mokra: "Anti-IL-8 antibody potentiates the effect of exogenous surfactant in respiratory failure caused by meconium aspiration." dans: Experimental lung research, Vol. 44, Issue 1, pp. 40-50, (2019) (PubMed).

Kopincova, Mikolka, Kolomaznik, Kosutova, Calkovska, Mokra: "Selective inhibition of NF-kappaB and surfactant therapy in experimental meconium-induced lung injury." dans: Physiological research, Vol. 66, Issue Suppl 2, pp. S227-S236, (2018) (PubMed).

Ma, Zhu, Chen, Zhang, Dong, Wu, Lu, Wu: "Sulforaphane, a Natural Isothiocyanate Compound, Improves Cardiac Function and Remodeling by Inhibiting Oxidative Stress and Inflammation in a Rabbit Model of Chronic Heart Failure." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1473-1483, (2018) (PubMed).

Li, Yang, Bai, Zhou, Semenza, Ge: "Chronic cold exposure results in subcutaneous adipose tissue browning and altered global metabolism in Qinghai-Tibetan plateau pika (Ochotona curzoniae)." dans: Biochemical and biophysical research communications, Vol. 500, Issue 2, pp. 117-123, (2018) (PubMed).

Kopincova, Mikolka, Kolomaznik, Kosutova, Calkovska, Mokra: "Modified porcine surfactant enriched by recombinant human superoxide dismutase for experimental meconium aspiration syndrome." dans: Life sciences, Vol. 203, pp. 121-128, (2018) (PubMed).

Mokra, Kosutova, Balentova, Adamkov, Mikolka, Mokry, Antosova, Calkovska: "Effects of budesonide on the lung functions, inflammation and apoptosis in a saline-lavage model of acute lung injury." dans: Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, Vol. 67, Issue 6, pp. 919-932, (2017) (PubMed).

Liu, Pei, Wang, Li, Zhao, Song, Chen, Liu: "The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis." dans: Oncotarget, Vol. 8, Issue 39, pp. 66528-66539, (2017) (PubMed).

Sozański, Kucharska, Rapak, Szumny, Trocha, Merwid-Ląd, Dzimira, Piasecki, Piórecki, Magdalan, Szeląg: "Iridoid-loganic acid versus anthocyanins from the Cornus mas fruits (cornelian cherry): Common and different effects on diet-induced atherosclerosis, PPARs expression and inflammation." dans: Atherosclerosis, Vol. 254, pp. 151-160, (2016) (PubMed).

Liu, Peng, Fang, Yao, Redmond, Ni: "No midterm advantages in the middle term using small intestinal submucosa and human amniotic membrane in Achilles tendon transverse tenotomy." dans: Journal of orthopaedic surgery and research, Vol. 11, Issue 1, pp. 125, (2016) (PubMed).

Mikolka, Kopincová, Košútová, Čierny, Čalkovská, Mokrá: "Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome." dans: Physiological research, Vol. 65, Issue Supplementum 5, pp. S653-S662, (2016) (PubMed).

Yu, Xie, Xin, Wang: "Panax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERK." dans: International journal of clinical and experimental pathology, Vol. 8, Issue 1, pp. 441-9, (2015) (PubMed).

Castellani, Laufer, Melka, Diehl, OReilly, Singh: "DNA methylation differences in monozygotic twin pairs discordant for schizophrenia identifies psychosis related genes and networks." dans: BMC medical genomics, Vol. 8, pp. 17, (2015) (PubMed).

Lin, Chen, Lei, You, Huang, Luo, Li, Zhao, Yan: "Effects of Intravenous Injection of Porphyromonas gingivalis on Rabbit Inflammatory Immune Response and Atherosclerosis." dans: Mediators of inflammation, Vol. 2015, pp. 364391, (2015) (PubMed).

Marques, Teixeira, Aguas, Ribeiro, Costa-e-Silva, Ferreira: "Immunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD)." dans: Veterinary research, Vol. 45, pp. 14, (2014) (PubMed).

Chen, Lin, You, Lei, Li, Lin, Luo, Yan: "Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: implications in acute and chronic infections." dans: Archives of oral biology, Vol. 59, Issue 10, pp. 1075-84, (2014) (PubMed).

Fang, Ning, Waqar, Niimi, Li, Satoh, Shiomi, Ye, Dong, Fan: "Bisphenol A exposure enhances atherosclerosis in WHHL rabbits." dans: PLoS ONE, Vol. 9, Issue 10, pp. e110977, (2014) (PubMed).

Hoekstra, van Lienden, Verheij, van der Loos, Heger, van Gulik: "Enhanced tumor growth after portal vein embolization in a rabbit tumor model." dans: The Journal of surgical research, Vol. 180, Issue 1, pp. 89-96, (2013) (PubMed).

Sakuramoto, Shimojo, Jesmin, Unoki, Kamiyama, Oki, Miya, Kawano, Mizutani: "Repeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS." dans: BMC anesthesiology, Vol. 13, Issue 1, pp. 47, (2013) (PubMed).

Moon, Joe, Kwon, Choi, Park, Kim: "Notochordal cells influence gene expression of inflammatory mediators of annulus fibrosus cells in proinflammatory cytokines stimulation." dans: Journal of Korean Neurosurgical Society, Vol. 48, Issue 1, pp. 1-7, (2010) (PubMed).

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Abstract: IL6 Produits

Synonymes: BSF2 Kit ELISA, HGF Kit ELISA, HSF Kit ELISA, IFNB2 Kit ELISA, IL-6 Kit ELISA, Il-6 Kit ELISA, ILg6 Kit ELISA, Ifnb2 Kit ELISA, il6 Kit ELISA, CHIL-6 Kit ELISA, interleukin 6 Kit ELISA, interleukin-6 Kit ELISA, IL6 Kit ELISA, Il6 Kit ELISA, il-6 Kit ELISA, IL-6 Kit ELISA

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome