MCM5 Kit ELISA

N° du produit ABIN6957842

Détail du produit MCM5 Kit ELISA

Antigène: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MCM5 in human tissue homogenates.

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Minichromosome Maintenance Deficient 5 (MCM5)

Sensibilité: 0.061 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 10 ng/mL. Prepare 7 tubes containing 0.5 mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0 ng/mL.
3. Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with Assay Diluent A and B, respectively (1:100).
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for once pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Stock: 4 °C/-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Date de péremption: 6 months

Détail du antigène

Antigène: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Autre désignation: Minichromosome Maintenance Deficient 5 (MCM5) (MCM5 Produits)

Synonymes: 23.m06024 Kit ELISA, cdc46 Kit ELISA, xmcm5 Kit ELISA, CDC46 Kit ELISA, P1-CDC46 Kit ELISA, Afu5g02520 Kit ELISA, NCU01171.1 Kit ELISA, AA617332 Kit ELISA, AI324988 Kit ELISA, AL033333 Kit ELISA, Cdc46 Kit ELISA, Mcmd5 Kit ELISA, mCD46 Kit ELISA, mCDC46 Kit ELISA, mcm5 Kit ELISA, wu:fb34a06 Kit ELISA, CG4082 Kit ELISA, DmCDC46 Kit ELISA, DmCDC465 Kit ELISA, DmMCM5 Kit ELISA, DmMcm5 Kit ELISA, DmeMCM5 Kit ELISA, Dmel\\CG4082 Kit ELISA, MCM5 Kit ELISA, McM5 Kit ELISA, PCR4 Kit ELISA, DNA replication licensing factor MCM5 Kit ELISA, minichromosome maintenance complex component 5 S homeolog Kit ELISA, minichromosome maintenance complex component 5 Kit ELISA, DNA replication licensing factor Mcm5 Kit ELISA, DNA replication licensing factor mcm5 Kit ELISA, MCM5 minichromosome maintenance deficient 5 (S. cerevisiae) Kit ELISA, minichromosome maintenance complex component 5 L homeolog Kit ELISA, Minichromosome maintenance 5 Kit ELISA, DNA replication licensing factor mcm-5 Kit ELISA, BBOV_IV010040 Kit ELISA, mcm5.S Kit ELISA, MCM5 Kit ELISA, AFUA_5G02520 Kit ELISA, NCU01171 Kit ELISA, PVX_084615 Kit ELISA, LOC5576119 Kit ELISA, CpipJ_CPIJ008678 Kit ELISA, Bm1_24480 Kit ELISA, CMU_022890 Kit ELISA, Mcm5 Kit ELISA, mcm5 Kit ELISA, TP01_0722 Kit ELISA, mcm5.L Kit ELISA, mcm-5 Kit ELISA

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA