Osteocalcin Kit ELISA

N° du produit ABIN6958337

Détail du produit Osteocalcin Kit ELISA

Antigène: Osteocalcin (BGLAP)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of osteocalcin in rat serum, plasma, tissue homogenates, cell lysates.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC)

Sensibilité: 6.3 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 5,000pg/mL. Firstly dilute the stock solution to 1,000pg/mL and the diluted standard serves as the highest standard (1,000pg/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
6. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Stock: 4 °C/-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

.

Date de péremption: 6 months

Références pour Osteocalcin Kit ELISA (ABIN6958337)

Nowak, Matuszewska, Nikodem, Filipiak, Landwójtowicz, Sadanowicz, Jędrzejuk, Rzeszutko, Zduniak, Piasecki, Kowalski, Dziewiszek, Merwid-Ląd, Trocha, Sozański, Kwiatkowska, Bolanowski, Szeląg: "Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopenia." dans: Pharmacological reports : PR, Vol. 69, Issue 5, pp. 1113-1119, (2018) (PubMed).

Liu, Xiong, Wang, Yang, Wang, Liu, Wu, Li, Ou, Zhang, Zhu: "Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model." dans: Journal of ethnopharmacology, Vol. 221, pp. 126-136, (2018) (PubMed).

Chen, Xue, Shen, Mu, Fu: "Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway." dans: International journal of molecular medicine, Vol. 37, Issue 2, pp. 329-38, (2016) (PubMed).

de Cássia Alves Nunes, Chiba, Pereira, Pereira, de Lima Coutinho Mattera, Ervolino, Louzada, Buzalaf, Silva, Sumida: "Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signaling and Insulin Sensitivity in Ovariectomized Rats." dans: Biological trace element research, Vol. 173, Issue 1, pp. 144-53, (2016) (PubMed).

Chen, Wang, Wang, Yin, Gao, Xiang, Liu, Xiong, Wang, Zhu, Yang, Zhang: "Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway." dans: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 279-287, (2016) (PubMed).

Nowak, Matuszewska, Filipiak, Nikodem, Merwid-Ląd, Pieśniewska, Fereniec-Gołębiewska, Kwiatkowska, Szeląg: "The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in rats." dans: Advances in medical sciences, Vol. 61, Issue 1, pp. 85-9, (2016) (PubMed).

Stringhetta-Garcia, Singulani, Santos, Louzada, Nakamune, Chaves-Neto, Rossi, Ervolino, Dornelles: "The effects of strength training and raloxifene on bone health in aging ovariectomized rats." dans: Bone, Vol. 85, pp. 45-54, (2016) (PubMed).

Zhang, Chen, Wei, Liu, Lei, Bai: "Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway." dans: Chemico-biological interactions, Vol. 256, pp. 188-97, (2016) (PubMed).

Matuszewska, Nowak, Rzeszutko, Zduniak, Szandruk, Jędrzejuk, Landwójtowicz, Bolanowski, Pieśniewska, Kwiatkowska, Szeląg: "Effects of long-term administration of pantoprazole on bone mineral density in young male rats." dans: Pharmacological reports : PR, Vol. 68, Issue 5, pp. 1060-4, (2016) (PubMed).

Shalan, Mustapha, Mohamed: "Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats." dans: Nutrition (Burbank, Los Angeles County, Calif.), Vol. 33, pp. 42-51, (2016) (PubMed).

Pereira, Chiba, de Lima Coutinho Mattera, Pereira, de Cássia Alves Nunes, Tsosura, Okamoto, Sumida: "Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats." dans: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), Vol. 39, pp. 140-146, (2016) (PubMed).

Chen, Li, Kong, Zhang, Lee: "Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation." dans: International journal of nanomedicine, Vol. 10, pp. 283-95, (2015) (PubMed).

Fauzi Kamal, Hadisoebroto Dilogo, Untung Hutagalung, Iskandriati, Susworo, Chaerani Siregar, Aulia Yusuf, Bachtiar et al.: "Transplantation of mesenchymal stem cells, recombinant human BMP-2,and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally ..." dans: Medical journal of the Islamic Republic of Iran, Vol. 28, pp. 129, (2015) (PubMed).

Nascimento da Silva, Azevedo de Jesuz, De Salvo Castro, Soares da Costa, Teles Boaventura, Blondet de Azeredo: "Effect of the “protein diet” and bone tissue." dans: Nutrición hospitalaria, Vol. 29, Issue 1, pp. 140-5, (2014) (PubMed).

Aydin, Halici, Akoz, Karaman, Ferah, Bayir, Aksakal, Akpinar, Selli, Kovaci: "Treatment with ?-lipoic acid enhances the bone healing after femoral fracture model of rats." dans: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 387, Issue 11, pp. 1025-36, (2014) (PubMed).

Kim, Lee, Jo, Song, Lim, Park, Bonewald, Kim: "Exendin-4 increases bone mineral density in type 2 diabetic OLETF rats potentially through the down-regulation of SOST/sclerostin in osteocytes." dans: Life sciences, Vol. 92, Issue 10, pp. 533-40, (2013) (PubMed).

Colli, Okamoto, Spritzer, Dornelles: "Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats." dans: Archives of oral biology, Vol. 57, Issue 9, pp. 1290-7, (2012) (PubMed).

Alm??an, B?ciu?, B?ciu?, Alm??an, Bran, Oana: "Serum changes induced by intramedullar experimental administration of bisphosphonates." dans: Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, Vol. 52, Issue 1 Suppl, pp. 435-42, (2011) (PubMed).

Détail du antigène

Antigène: Osteocalcin (BGLAP)

Autre désignation: Osteocalcin (OC) (BGLAP Produits)

Synonymes: BGP Kit ELISA, OC Kit ELISA, OCN Kit ELISA, Bglap1 Kit ELISA, Bglap2 Kit ELISA, OG1 Kit ELISA, mOC-A Kit ELISA, Bgp Kit ELISA, Bgpr Kit ELISA, Bgpra Kit ELISA, bgp Kit ELISA, LOC792433 Kit ELISA, ocn Kit ELISA, GLA Kit ELISA, BGLAP Kit ELISA, PMF1 Kit ELISA, bone gamma-carboxyglutamate protein Kit ELISA, bone gamma carboxyglutamate protein Kit ELISA, bone gamma-carboxyglutamate protein S homeolog Kit ELISA, bone gamma-carboxyglutamate (gla) protein Kit ELISA, osteocalcin Kit ELISA, polyamine-modulated factor 1 Kit ELISA, BGLAP Kit ELISA, Bglap Kit ELISA, bglap.S Kit ELISA, bglap Kit ELISA, ocn Kit ELISA, LOC100529177 Kit ELISA, LOC100146589 Kit ELISA