TNF alpha Kit ELISA

N° du produit ABIN6963447

Détail du produit TNF alpha Kit ELISA

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes Human TNF-α in samples. No Significant cross-reactivity or interference between Human TNF-α and analogues was observed.

Sensibilité: 4.69 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 500 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 500, 250, 125, 62.5, 31.25, 15.63, 7.81, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 500 pg/mL working solution to the first tube and mix up to produce a 250 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TNF-α were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 12 months

Références pour TNF alpha Kit ELISA (ABIN6963447)

Ilmiawati, Fitri, Rofinda, Reza: "Green coffee extract modifies body weight, serum lipids and TNF-α in high-fat diet-induced obese rats." dans: BMC research notes, Vol. 13, Issue 1, pp. 208, (2021) (PubMed).

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Liu, Zhang, Luo, Qin, Yang, Wei: "iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin-angiotensin system-mediated congenital ureteropelvic junction obstruction." dans: Experimental cell research, Vol. 393, Issue 1, pp. 112086, (2021) (PubMed).

Al-Hakeim, Najm, Al-Dujaili, Maes: "Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased Pro-inflammatory Cytokines." dans: Neurotoxicity research, Vol. 38, Issue 1, pp. 228-241, (2021) (PubMed).

Shen, Zheng, Wang, Hu, Jiang, Jiang: "LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD." dans: Biomedicine & pharmacotherapy, Vol. 126, pp. 110016, (2021) (PubMed).

Zhang, Lv, Du: "Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 26, pp. e921957, (2021) (PubMed).

Sun, Cai, Shen, Jin, Xie: "Correlation Between Single Nucleotide Polymorphisms at the 3'-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis." dans: Genetic testing and molecular biomarkers, Vol. 24, Issue 5, pp. 274-284, (2021) (PubMed).

Rakhra, Masih, Vats, Vijay, Ashraf, Singh: "Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line." dans: Biological trace element research, Vol. 195, Issue 1, pp. 95-104, (2021) (PubMed).

Zhou, Huang, Xu, Zhou, Shao, Song: "Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1." dans: Archives of oral biology, Vol. 118, pp. 104858, (2021) (PubMed).

Yao, Zhao, Tan, Cao, Guo, Li, Huang, Diabakte, Wang, Liu, Shen, Li, Cao, Sheng, Lu, Cao, Jin, Zhang, Tian: "Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits." dans: Scientific reports, Vol. 10, Issue 1, pp. 11638, (2021) (PubMed).

Jiang, Liu, Wu, Wu, Lu, Xu, Gao, Zhao: "Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology." dans: Journal of ethnopharmacology, Vol. 249, pp. 112433, (2020) (PubMed).

Maharshi, Gupta, Kumar, Upadhyay, Das, Yadav, Nayak, Kumar, Shalimar: "Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized-controlled pilot study." dans: Gastroenterology report, Vol. 8, Issue 2, pp. 104-110, (2020) (PubMed).

Zhao, Liu, Deng, Ma, Liao, Liang, Hu, Li, Guo, Cai, Xu, Gao, Su: "Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output." dans: Cell, Vol. 183, Issue 1, pp. 76-93.e22, (2020) (PubMed).

Liu, Zhou, Niu, Pu, Wang, Huang, Zhao, Yang, Tao, Xia, Feng: "Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF-α-mediated osteoclast autophagy." dans: Experimental and therapeutic medicine, Vol. 20, Issue 3, pp. 1889-1898, (2020) (PubMed).

Iyigun, Alikasifoglu, Gonc, Ozon, Eryilmaz Polat, Hizal, Kiper, Ozcelik: "Obstructive sleep apnea in children with hypothalamic obesity: Evaluation of possible related factors." dans: Pediatric pulmonology, Vol. 55, Issue 12, pp. 3532-3540, (2020) (PubMed).

Li, Li, Yang, Wang: "Human cytomegalovirus infection is correlated with atherosclerotic plaque vulnerability in carotid artery." dans: The journal of gene medicine, Vol. 22, Issue 10, pp. e3236, (2020) (PubMed).

Li, Wu, Chen, Jiang, Ren: "Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota." dans: Fish & shellfish immunology, Vol. 104, pp. 622-632, (2020) (PubMed).

Li, Ye, Gao, Cheng: "Plasma concentration of ZCCHC14 contributes to prognostic efficacy in intracerebral hemorrhage patients." dans: European review for medical and pharmacological sciences, Vol. 23, Issue 19, pp. 8533-8539, (2020) (PubMed).

Almulla, Moustafa, Al-Dujaili, Al-Hakeim, Maes: "Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments." dans: Revista brasileira de psiquiatria (Sao Paulo, Brazil : 1999), (2020) (PubMed).

Cao, Cao, Liu, Wang, He, Xu, Wang: "Effect of laparoscopic sleeve gastrectomy on renal function in obese patients." dans: ANZ journal of surgery, Vol. 90, Issue 4, pp. 514-520, (2020) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor Alpha (TNF alpha Produits)

Synonymes: DIF Kit ELISA, TNF-alpha Kit ELISA, TNFA Kit ELISA, TNFSF2 Kit ELISA, RATTNF Kit ELISA, Tnfa Kit ELISA, tnf Kit ELISA, TNF-a Kit ELISA, TNFalpha Kit ELISA, Tnfsf1a Kit ELISA, TNFa Kit ELISA, cTNF Kit ELISA, Tnf-alpha Kit ELISA, tnfa-like Kit ELISA, TNF-ALPHA Kit ELISA, dif Kit ELISA, tnfa Kit ELISA, xtnf Kit ELISA, tnfsf2 Kit ELISA, tnf-alpha Kit ELISA, Cachectin Kit ELISA, tumor necrosis factor Kit ELISA, tumor necrosis factor b (TNF superfamily, member 2) Kit ELISA, tumor necrosis factor alpha Kit ELISA, tumor necrosis factor a (TNF superfamily, member 2) Kit ELISA, TNF Kit ELISA, Tnf Kit ELISA, tnf Kit ELISA, tnfb Kit ELISA, tnf-alpha Kit ELISA, LOC103694380 Kit ELISA, tnfa Kit ELISA

Sujet: DIF, TNF-alpha, TNFA, TNFSF2

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome