HSPA9 Kit ELISA

N° du produit ABIN6963529

Détail du produit HSPA9 Kit ELISA

Antigène: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes Human HSP-70/HSPA9 in samples. No Significant cross-reactivity or interference between Human HSP-70/HSPA9 and analogues was observed.

Sensibilité: 0.47 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 50 ng/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 50, 25, 12.5, 6.25, 3.13, 1.56, 0.78, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 50 ng/mL working solution to the first tube and mix up to produce a 25 ng/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human HSP-70/HSPA9 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human HSP-70/HSPA9 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 12 months

Références pour HSPA9 Kit ELISA (ABIN6963529)

Li, Liu, Zang, Zhou, Zhang, Sun, Qi, Li, Kong, Jin, Yang, Luo, Lu, Lin, Niu, Liu: "Small extracellular vesicle-bound vascular endothelial growth factor secreted by carcinoma-associated fibroblasts promotes angiogenesis in a bevacizumab-resistant manner." dans: Cancer letters, Vol. 492, pp. 71-83, (2021) (PubMed).

Yuan, Dai, Li, Zheng, Zhao, Dong: "Bexarotene Improve Depression-Like Behaviour in Mice by Protecting Against Neuro-inflammation and Synaptic Damage." dans: Neurochemical research, Vol. 45, Issue 7, pp. 1500-1509, (2020) (PubMed).

Détail du antigène

Antigène: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Autre désignation: Heat Shock 70 kDa Protein 9 (HSPA9 Produits)

Synonymes: APG-2 Kit ELISA, HS24/P52 Kit ELISA, HSPH2 Kit ELISA, RY Kit ELISA, hsp70 Kit ELISA, hsp70RY Kit ELISA, CSA Kit ELISA, GRP-75 Kit ELISA, GRP75 Kit ELISA, HSPA9B Kit ELISA, MOT Kit ELISA, MOT2 Kit ELISA, MTHSP75 Kit ELISA, PBP74 Kit ELISA, Hspa9a Kit ELISA, csa Kit ELISA, grp-75 Kit ELISA, grp75 Kit ELISA, hspa9 Kit ELISA, hspa9b Kit ELISA, mortalin Kit ELISA, mot Kit ELISA, mot2 Kit ELISA, pbp74 Kit ELISA, mot-2 Kit ELISA, mthsp75 Kit ELISA, 74kDa Kit ELISA, Csa Kit ELISA, Grp75 Kit ELISA, Hsc74 Kit ELISA, Hsp74 Kit ELISA, Hsp74a Kit ELISA, Mortalin Kit ELISA, Mot-2 Kit ELISA, Mot2 Kit ELISA, Mthsp70 Kit ELISA, Pbp74 Kit ELISA, cb740 Kit ELISA, crs Kit ELISA, wu:fc14d08 Kit ELISA, wu:fc27c10 Kit ELISA, wu:fc38a06 Kit ELISA, heat shock protein family A (Hsp70) member 4 Kit ELISA, heat shock protein family A (Hsp70) member 9 Kit ELISA, heat shock protein family A member 9 Kit ELISA, heat shock protein family A (Hsp70) member 9 S homeolog Kit ELISA, stress-70 protein, mitochondrial Kit ELISA, heat shock protein 9 Kit ELISA, heat shock protein Hsp9 Kit ELISA, HSPA4 Kit ELISA, HSPA9 Kit ELISA, Hspa9 Kit ELISA, hspa9.S Kit ELISA, hspa9 Kit ELISA, LOC577721 Kit ELISA, hsp9 Kit ELISA

Sujet: HSPA9, CSA, GRP-75, GRP75, HSPA9B, MOT, MOT2, MTHSP75, PBP74