alpha 2 Macroglobulin Kit ELISA

N° du produit ABIN6963561

Détail du produit alpha 2 Macroglobulin Kit ELISA

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 ng/mL - 4000 ng/mL

Seuil minimal de détection: 62.5 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes Human α2-M in samples. No significant cross-reactivity or interference between Human α2-M and analogues was observed.

Sensibilité: 37.5 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18-25 °C) before use. If the kit will not be used up in one assay, please only take out the necessary strips and reagents for present experiment, and store the remaining strips and reagents at required condition.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer. Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved.
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 4000 ng/mL(or add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 1-2 min and then mix it thoroughly with a vortex meter of low speed. Bubbles generated during vortex could be removed by centrifuging at a relatively low speed). Then make serial dilutions as needed. The recommended dilution gradient is as follows: 4000, 2000, 1000, 500, 250, 125, 62.5, 0 ng/mL. Dilution method: Take 7 EP tubes, add 500μL of Reference Standard & Sample Diluent to each tube. Pipette 500μL of the 4000 ng/mL working solution to the first tube and mix up to produce a 2000 ng/mL working solution. Pipette 500μL of the solution from the former tube into the latter one according to this step. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the Concentrated Biotinylated Detection Antibody at 800xg for 1 min, then dilute the 100x Concentrated Biotinylated Detection Antibody to 1x working solution with Biotinylated Detection Antibody Diluent(Concentrated Biotinylated Detection Antibody: Biotinylated Detection Antibody Diluent= 1: 99).
5. HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the Concentrated HRP Conjugate at 800xg for 1 min, then dilute -6- the 100x Concentrated HRP Conjugate to 1x working solution with HRP Conjugate Diluent(Concentrated HRP Conjugate: HRP Conjugate Diluent= 1: 99). 4000 2000 1000 500 250 125 62.5 0

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human α2-M were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human α2-M were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 12 months

Détail du antigène

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Autre désignation: Alpha-2 Macroglobulin (A2M Produits)

Synonymes: A2MD Kit ELISA, CPAMD5 Kit ELISA, FWP007 Kit ELISA, S863-7 Kit ELISA, A2MAC1 Kit ELISA, A2m1 Kit ELISA, A2maa Kit ELISA, Mam Kit ELISA, A2mp Kit ELISA, A2M Kit ELISA, LOC733429 Kit ELISA, endod Kit ELISA, cpamd5 Kit ELISA, fwp007 Kit ELISA, s863-7 Kit ELISA, a2mb Kit ELISA, endodermin Kit ELISA, Alpha-2-M Kit ELISA, alpha-2-macroglobulin Kit ELISA, pregnancy-zone protein L homeolog Kit ELISA, alpha-2-macroglobulin S homeolog Kit ELISA, pregnancy zone protein Kit ELISA, alpha-2-macroglobulin-like Kit ELISA, A2M Kit ELISA, A2m Kit ELISA, LOC477699 Kit ELISA, pzp.L Kit ELISA, AZL_c00450 Kit ELISA, LOC100349077 Kit ELISA, a2m Kit ELISA, LOC100061656 Kit ELISA, LOC100090399 Kit ELISA, a2m.S Kit ELISA, LOC100469973 Kit ELISA, LOC100595735 Kit ELISA, PZP Kit ELISA, LOC100353095 Kit ELISA, LOC101122940 Kit ELISA, LOC101801552 Kit ELISA, LOC100911545 Kit ELISA

Sujet: α2M, A2M

Pathways: Lipid Metabolism