IL-6 Kit ELISA
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- Antigène Voir toutes IL-6 (IL6) Kits ELISA
- IL-6 (IL6) (Interleukin 6 (IL6))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.25 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.25 pg/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This kit recognizes Mouse IL-6 in samples. No Significant cross-reactivity or interference between Mouse IL-6 and analogues was observed.
- Sensibilité
- 18.75 pg/mL
- Ingrédients
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- Pre-coated, ready to use 96-well strip plate, flat buttom
- Plate sealer for 96 wells
- Reference Standard
- Reference Standard & Sample Diluent
- Biotinylated Detection Antibody (100 x concentrate)
- HRP Conjugate (100 x concentrate)
- Biotinylated Detection Antibody Diluent
- HRP Conjugate Diluent
- Substrate Reagent
- Stop Solution
- Wash Buffer (25 x concentrate)
- Instruction manual
- Featured
- Discover our best selling IL6 Kit ELISA
- Top Product
- Discover our top product IL6 Kit ELISA
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- Volume d'échantillon
- 100 μL
- Durée du test
- 3.5 h
- Plaque
- Pre-coated
- Protocole
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- Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
- Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
- Aspirate and wash 3 times.
- Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
- Aspirate and wash 5 times.
- Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
- Add 50 µL Stop Solution. Read at 450 nm immediately.
- Calculation of results.
- Préparation des réactifs
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- Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
- Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
- Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 2000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 2000, 1000, 500, 250, 125, 62.5, 31.25, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 2000 pg/mL working solution to the first tube and mix up to produce a 1000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
- Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
- Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
- Préparation de l'échantillon
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- It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
- If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
- If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
- Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-6 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-6 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %. - Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
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- For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
- For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.
- Date de péremption
- 12 months
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Bifidobacteria-Fermented Red Ginseng and Its Constituents Ginsenoside Rd and Protopanaxatriol Alleviate Anxiety/Depression in Mice by the Amelioration of Gut Dysbiosis." dans: Nutrients, Vol. 12, Issue 4, (2021) (PubMed).
: "The Inhibition of P-Selectin Reduced Severe Acute Lung Injury in Immunocompromised Mice." dans: Oxidative medicine and cellular longevity, Vol. 2020, pp. 8430465, (2021) (PubMed).
: "Modulation of Gut Microbiota by Fucoxanthin During Alleviation of Obesity in High-Fat Diet-Fed Mice." dans: Journal of agricultural and food chemistry, Vol. 68, Issue 18, pp. 5118-5128, (2021) (PubMed).
: "Novel Biomedical Functions of Surfactin A from Bacillus subtilis in Wound Healing Promotion and Scar Inhibition." dans: Journal of agricultural and food chemistry, Vol. 68, Issue 26, pp. 6987-6997, (2021) (PubMed).
: "MG-132 attenuates cardiac deterioration of viral myocarditis via AMPK pathway." dans: Biomedicine & pharmacotherapy, Vol. 126, pp. 110091, (2021) (PubMed).
: "Molecular hydrogen attenuates sepsis-induced neuroinflammation through regulation of microglia polarization through an mTOR-autophagy-dependent pathway." dans: International immunopharmacology, Vol. 81, pp. 106287, (2021) (PubMed).
: "Antineuroinflammatory Effects of 7,3',4'-Trihydroxyisoflavone in Lipopolysaccharide-Stimulated BV2 Microglial Cells through MAPK and NF-κB Signaling Suppression." dans: Biomolecules & therapeutics, Vol. 29, Issue 2, pp. 127-134, (2021) (PubMed).
: "Saikosaponin-d attenuated lipopolysaccharide-induced depressive-like behaviors via inhibiting microglia activation and neuroinflammation." dans: International immunopharmacology, Vol. 80, pp. 106181, (2021) (PubMed).
: "Development, optimisation and evaluation of chitosan nanoparticles of alendronate against Alzheimer's disease in intracerebroventricular streptozotocin model for brain delivery." dans: Journal of drug targeting, Vol. 29, Issue 2, pp. 199-216, (2021) (PubMed).
: "Inhibition of PDE4/PDE4B improves renal function and ameliorates inflammation in cisplatin-induced acute kidney injury." dans: American journal of physiology. Renal physiology, Vol. 318, Issue 3, pp. F576-F588, (2020) (PubMed).
: "Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis." dans: Oxidative medicine and cellular longevity, Vol. 2019, pp. 1475729, (2020) (PubMed).
: "Circular RNA expression profile of liver tissues in an EtOH-induced mouse model of alcoholic hepatitis." dans: European journal of pharmacology, Vol. 862, pp. 172642, (2020) (PubMed).
: "Inflammatory kidney injury in trichloroethylene hypersensitivity syndrome mice: Possible role of C3a receptor in the accumulation of Th17 phenotype." dans: Ecotoxicology and environmental safety, Vol. 186, pp. 109772, (2020) (PubMed).
: "Maggot Extracts Alleviate Inflammation and Oxidative Stress in Acute Experimental Colitis via the Activation of Nrf2." dans: Oxidative medicine and cellular longevity, Vol. 2019, pp. 4703253, (2020) (PubMed).
: "Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway." dans: Current medical science, Vol. 39, Issue 5, pp. 748-753, (2020) (PubMed).
: "The Protective Effects of Melatonin Against LPS-Induced Septic Myocardial Injury: A Potential Role of AMPK-Mediated Autophagy." dans: Frontiers in endocrinology, Vol. 11, pp. 162, (2020) (PubMed).
: "Propacin, a coumarinolignoid isolated from durian, inhibits the lipopolysaccharide-induced inflammatory response in macrophages through the MAPK and NF-κB pathways." dans: Food & function, Vol. 11, Issue 1, pp. 596-605, (2020) (PubMed).
: "The protective effect of Geniposide on diabetic cognitive impairment through BTK/TLR4/NF-κB pathway." dans: Psychopharmacology, Vol. 237, Issue 2, pp. 465-477, (2020) (PubMed).
: "Camellia cake extracts reduce burn injury through suppressing inflammatory responses and enhancing collagen synthesis." dans: Food & nutrition research, Vol. 64, (2020) (PubMed).
: "Pregnane X receptor activation by its natural ligand Ginkgolide-A improves tight junction proteins expression and attenuates bacterial translocation in cirrhosis." dans: Chemico-biological interactions, Vol. 315, pp. 108891, (2020) (PubMed).
: "
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Bifidobacteria-Fermented Red Ginseng and Its Constituents Ginsenoside Rd and Protopanaxatriol Alleviate Anxiety/Depression in Mice by the Amelioration of Gut Dysbiosis." dans: Nutrients, Vol. 12, Issue 4, (2021) (PubMed).
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- Antigène Voir toutes IL-6 (IL6) Kits ELISA
- IL-6 (IL6) (Interleukin 6 (IL6))
- Autre désignation
- Interleukin 6 (IL6 Produits)
- Synonymes
- BSF2 Kit ELISA, HGF Kit ELISA, HSF Kit ELISA, IFNB2 Kit ELISA, IL-6 Kit ELISA, Il-6 Kit ELISA, ILg6 Kit ELISA, Ifnb2 Kit ELISA, il6 Kit ELISA, CHIL-6 Kit ELISA, interleukin 6 Kit ELISA, interleukin-6 Kit ELISA, IL6 Kit ELISA, Il6 Kit ELISA, il-6 Kit ELISA, IL-6 Kit ELISA
- Sujet
- IL6, BSF2, HGF, HSF, IFNB2, CDF
- Pathways
- Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
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