TNF alpha Kit ELISA

N° du produit ABIN6963666

Détail du produit TNF alpha Kit ELISA

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.13 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.13 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes Rat TNF-α in samples. No Significant cross-reactivity or interference between Rat TNF- α and analogues was observed.

Sensibilité: 46.88 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 5000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 5000, 2500, 1250, 625, 312.5, 156.25, 78.13, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 5000 pg/mL working solution to the first tube and mix up to produce a 2500 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat TNF-α were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 12 months

Références pour TNF alpha Kit ELISA (ABIN6963666)

Choi, Ham, Ryu, Lee, Miguel, Paik, Ji, Park, Kang, Lee, Lee: "Defatted Tenebrio molitor Larva Fermentation Extract Modifies Steatosis, Inflammation and Intestinal Microflora in Chronic Alcohol-Fed Rats." dans: Nutrients, Vol. 12, Issue 5, (2021) (PubMed).

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Adedara, Awogbindin, Maduako, Ajeleti, Owumi, Owoeye, Patlolla, Farombi: "Kolaviron suppresses dysfunctional reproductive axis associated with multi-walled carbon nanotubes exposure in male rats." dans: Environmental science and pollution research international, Vol. 28, Issue 1, pp. 354-364, (2021) (PubMed).

Abdelzaher, Abdel-Hafez, Rofaeil, Ali, Hegazy, Bahaa: "The protective effect of fenofibrate, triptorelin, and their combination against premature ovarian failure in rats." dans: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 394, Issue 1, pp. 137-149, (2021) (PubMed).

Adedara, Fabunmi, Ayenitaju, Atanda, Adebowale, Ajayi, Owoeye, Rocha, Farombi: "Neuroprotective mechanisms of selenium against arsenic-induced behavioral impairments in rats." dans: Neurotoxicology, Vol. 76, pp. 99-110, (2021) (PubMed).

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Chen, Zhang, Li, Liu, Wang, Zhang, Xu, Li, Guo, Yao: "Berberine regulates fecal metabolites to ameliorate 5-fluorouracil induced intestinal mucositis through modulating gut microbiota." dans: Biomedicine & pharmacotherapy, Vol. 124, pp. 109829, (2021) (PubMed).

Han, Chen, Chen, Luo, Liu, He, He, Qiu, Chen, Wu, Zhuang, Yang, Wu, Wu, Yang, Ge, Zhuang, Yu: "Tetramethylpyrazine attenuates endotoxin-induced retinal inflammation by inhibiting microglial activation via the TLR4/NF-κB signalling pathway." dans: Biomedicine & pharmacotherapy, Vol. 128, pp. 110273, (2021) (PubMed).

Liu, He, Wang, Wang, Sun, Li, Jia, Wang, Xu, Ming, Wang, Lin: "Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways." dans: Journal of ethnopharmacology, Vol. 260, pp. 113039, (2021) (PubMed).

Yang, Jia, Li, Mehmood: "Protective effect of exogenous hydrogen sulfide on diaphragm muscle fibrosis in streptozotocin-induced diabetic rats." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 245, Issue 14, pp. 1280-1289, (2021) (PubMed).

Song, Sun, Li, Long, Yan, Qin, Makinde, Famurewa, Jaisi, Nie, Olatunji: "Tiliacora triandra extract and its major constituent attenuates diabetic kidney and testicular impairment by modulating redox imbalance and pro-inflammatory responses in rats." dans: Journal of the science of food and agriculture, Vol. 101, Issue 4, pp. 1598-1608, (2021) (PubMed).

Gao, Hou, Wang, Guo, Yuan, Jiang, Duan, Zhang, Xu, Che, Sun, Li, Zhang, Sun, Li: "Octreotide alleviates pancreatic damage caused by paraquat in rats by reducing inflammatory responses and oxidative stress." dans: Environmental toxicology and pharmacology, Vol. 80, pp. 103456, (2021) (PubMed).

Owumi, Adedara, Farombi, Oyelere: "Protocatechuic acid modulates reproductive dysfunction linked to furan exposure in rats." dans: Toxicology, Vol. 442, pp. 152556, (2021) (PubMed).

Abdel-Aziz, Naguib Abdel Hafez: "Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM-1 and VEGF-A." dans: Andrologia, Vol. 52, Issue 2, pp. e13472, (2020) (PubMed).

Alzahrani, Alim A Sattar, Kamel, Ramadan, Alzahrani: "Possible combined effect of perindopril and Azilsartan in an experimental model of dementia in rats." dans: Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, Vol. 28, Issue 5, pp. 574-581, (2020) (PubMed).

Durmaz, Kurtoğlu, Barbarus, Eliyatkın, Yılmaz: "TNF-alpha inhibitor adalimumab attenuates endotoxin induced cardiac damage in rats." dans: Acta cirurgica brasileira, Vol. 35, Issue 2, pp. e202000202, (2020) (PubMed).

Rabie, Abd El Fattah, Nassar, Abdallah, El-Abhar et al.: "Correlation between angiotensin 1-7-mediated Mas receptor expression with motor improvement, activated STAT3/SOCS3 cascade, and suppressed HMGB-1/RAGE/NF-κB signaling in 6-hydroxydopamine ..." dans: Biochemical pharmacology, Vol. 171, pp. 113681, (2020) (PubMed).

Sayan, Karabulut, Özdamar: "Assessment of the protective and therapeutic effect of melatonin against thioacetamide-induced acute liver damage." dans: Journal of biochemical and molecular toxicology, Vol. 34, Issue 4, pp. e22450, (2020) (PubMed).

Adedara, Awogbindin, Owoeye, Maduako, Ajeleti, Owumi, Patlolla, Farombi: "Kolaviron via anti-inflammatory and redox regulatory mechanisms abates multi-walled carbon nanotubes-induced neurobehavioral deficits in rats." dans: Psychopharmacology, Vol. 237, Issue 4, pp. 1027-1040, (2020) (PubMed).

Awogbindin, Maduako, Adedara, Owumi, Ajeleti, Owoeye, Patlolla, Tchounwou, Farombi: "Kolaviron ameliorates hepatic and renal dysfunction associated with multiwalled carbon nanotubes in rats." dans: Environmental toxicology, (2020) (PubMed).

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: Tumor Necrosis Factor Alpha (TNF alpha Produits)

Synonymes: DIF Kit ELISA, TNF-alpha Kit ELISA, TNFA Kit ELISA, TNFSF2 Kit ELISA, RATTNF Kit ELISA, Tnfa Kit ELISA, tnf Kit ELISA, TNF-a Kit ELISA, TNFalpha Kit ELISA, Tnfsf1a Kit ELISA, TNFa Kit ELISA, cTNF Kit ELISA, Tnf-alpha Kit ELISA, tnfa-like Kit ELISA, TNF-ALPHA Kit ELISA, dif Kit ELISA, tnfa Kit ELISA, xtnf Kit ELISA, tnfsf2 Kit ELISA, tnf-alpha Kit ELISA, Cachectin Kit ELISA, tumor necrosis factor Kit ELISA, tumor necrosis factor b (TNF superfamily, member 2) Kit ELISA, tumor necrosis factor alpha Kit ELISA, tumor necrosis factor a (TNF superfamily, member 2) Kit ELISA, TNF Kit ELISA, Tnf Kit ELISA, tnf Kit ELISA, tnfb Kit ELISA, tnf-alpha Kit ELISA, LOC103694380 Kit ELISA, tnfa Kit ELISA

Sujet: DIF, TNF-alpha, TNFA, TNFSF2

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome