HSP90 Kit ELISA

N° du produit ABIN6963691

Détail du produit HSP90 Kit ELISA

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes Rat HSP-90 in samples. No Significant cross-reactivity or interference between Rat HSP-90 and analogues was observed.

Sensibilité: 75 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3.5 h

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 8000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 8000, 4000, 2000, 1000, 500, 250, 125.00, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 8000 pg/mL working solution to the first tube and mix up to produce a 4000 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat HSP-90 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat HSP-90 were tested on 3 different plates, 20 replicates in each plate.
Both intra-CV and inter-CV are < 10 %.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 12 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Date de péremption: 12 months

Détail du antigène

Antigène: HSP90 (Heat Shock Protein 90 (HSP90))

Autre désignation: Heat Shock Protein 90 (HSP90 Produits)

Synonymes: EL52 Kit ELISA, HSP86 Kit ELISA, HSP89A Kit ELISA, HSP90A Kit ELISA, HSP90N Kit ELISA, HSPC1 Kit ELISA, HSPCA Kit ELISA, HSPCAL1 Kit ELISA, HSPCAL4 Kit ELISA, HSPN Kit ELISA, Hsp89 Kit ELISA, Hsp90 Kit ELISA, LAP2 Kit ELISA, git10 Kit ELISA, swo1 Kit ELISA, HSP90 Kit ELISA, htpG Kit ELISA, SCBAC25F8.08 Kit ELISA, 23.m06066 Kit ELISA, 17.m07646 Kit ELISA, HSP90-1 Kit ELISA, 143198_at Kit ELISA, 83 Kit ELISA, 83K HSP Kit ELISA, DMHSP82 Kit ELISA, E(sev)3A Kit ELISA, E(sina)2 Kit ELISA, HSP82 Kit ELISA, HSP83 Kit ELISA, ORF1 Kit ELISA, Su(Raf)3A Kit ELISA, anon-EST:Liang-2.53 Kit ELISA, anon-WO0068693 Kit ELISA, anon-WO0140519.209 Kit ELISA, clone 2.53 Kit ELISA, en(lz)3C/4C Kit ELISA, hsp84 Kit ELISA, l(3)j5C2 Kit ELISA, ms(3)08445 Kit ELISA, stc Kit ELISA, DmelCG1242 Kit ELISA, CG1242 Kit ELISA, Hsp86 Kit ELISA, Hspca Kit ELISA, 86kDa Kit ELISA, 89kDa Kit ELISA, AL024080 Kit ELISA, AL024147 Kit ELISA, Hsp86-1 Kit ELISA, hsp4 Kit ELISA, hsp86 Kit ELISA, hsp89 Kit ELISA, hsp90 Kit ELISA, hsp90a Kit ELISA, hspc1 Kit ELISA, hspca Kit ELISA, hspn Kit ELISA, lap2 Kit ELISA, Hsp90alpha Kit ELISA, heat shock protein 90 alpha family class A member 1 Kit ELISA, heat shock protein Hsp90 Kit ELISA, Hsp90 chaperone Kit ELISA, Heat shock protein 90 Kit ELISA, heat shock protein 90 Kit ELISA, chaperone protein HtpG Kit ELISA, molecular chaperone HtpG Kit ELISA, Heat Shock Protein 90 Kit ELISA, uncharacterized LOC100384473 Kit ELISA, Heat shock protein 83 Kit ELISA, heat shock protein 90, alpha (cytosolic), class A member 1 Kit ELISA, heat shock protein 90 alpha family class B member 1 Kit ELISA, heat shock protein 90kDa alpha family class A member 1 L homeolog Kit ELISA, heat shock protein HSP 90-alpha Kit ELISA, HSP90AA1 Kit ELISA, hsp90 Kit ELISA, HSP90 Kit ELISA, daf-21 Kit ELISA, htpG Kit ELISA, SCO7516 Kit ELISA, MAP_RS10510 Kit ELISA, TP04_0646 Kit ELISA, TP01_0934 Kit ELISA, APH_RS03525 Kit ELISA, GbCGDNIH1_0315 Kit ELISA, MAV_2118 Kit ELISA, HSP90C Kit ELISA, BBOV_IV008400 Kit ELISA, BBOV_III007380 Kit ELISA, ACICU_00312 Kit ELISA, ECL_01244 Kit ELISA, YE105_C1172 Kit ELISA, pco153543(105) Kit ELISA, Hsp83 Kit ELISA, Hsp90aa1 Kit ELISA, HSP90AB1 Kit ELISA, hsp90aa1.1.L Kit ELISA, LOC108698781 Kit ELISA

Sujet: Hsp90

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals