beta Actin Kit ELISA

N° du produit ABIN6973738

Détail du produit beta Actin Kit ELISA

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Fonction: For the quantitative determination of human β-actin concentrations in serum, plasma, tissue homogenates, cell lysates.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of human β-actin. No significant cross-reactivity or interference between human β-actin and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human β-actin and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.078 ng/mL

Ingrédients:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Préparation des réactifs:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 20 ng/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undi luted Standard serves as the high standard (20 ng/mL). Sample Diluent serves as the zero standard (0 ng/mL).

Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

Date de péremption: 6 months

Détail du antigène

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: actin, beta (ACTB Produits)

Synonymes: BRWS1 Kit ELISA, PS1TP5BP1 Kit ELISA, ACTA1 Kit ELISA, Actx Kit ELISA, E430023M04Rik Kit ELISA, beta-actin Kit ELISA, ps1tp5bp1 Kit ELISA, act-2 Kit ELISA, actb Kit ELISA, B-actin Kit ELISA, LOC100174883 Kit ELISA, LOC100304816 Kit ELISA, A Kit ELISA, ACT5C Kit ELISA, Ac5C Kit ELISA, Act Kit ELISA, Act-5C Kit ELISA, Act5 Kit ELISA, Act5c Kit ELISA, Actin Kit ELISA, Actin/BAP47 Kit ELISA, Actin5C Kit ELISA, BAP47 Kit ELISA, Bap47 Kit ELISA, CG4027 Kit ELISA, Dmel\\CG4027 Kit ELISA, M32055 Kit ELISA, T11 Kit ELISA, act Kit ELISA, act 5C Kit ELISA, act5C Kit ELISA, actin Kit ELISA, actin5C Kit ELISA, anon-EST:fe2D2 Kit ELISA, beta-actin/Bap47 Kit ELISA, cyt5C Kit ELISA, l(1)G0009 Kit ELISA, l(1)G0010 Kit ELISA, l(1)G0025 Kit ELISA, l(1)G0079 Kit ELISA, l(1)G0117 Kit ELISA, l(1)G0177 Kit ELISA, l(1)G0245 Kit ELISA, l(1)G0330 Kit ELISA, l(1)G0420 Kit ELISA, l(1)G0486 Kit ELISA, Bact Kit ELISA, ACT-5 Kit ELISA, ACTB Kit ELISA, B-ACTZF Kit ELISA, actba Kit ELISA, bact Kit ELISA, bactin1 Kit ELISA, bactzf Kit ELISA, wu:fd18f05 Kit ELISA, A26C1B Kit ELISA, POTE2alpha Kit ELISA, POTEACTIN Kit ELISA, Beta-actin Kit ELISA, actin beta Kit ELISA, actin, beta Kit ELISA, cytoplasmic actin OlCA1 Kit ELISA, beta-actin Kit ELISA, Actin 5C Kit ELISA, actin gamma 1 Kit ELISA, actin, beta 1 Kit ELISA, actin, beta L homeolog Kit ELISA, beta actin Kit ELISA, POTE ankyrin domain family member F Kit ELISA, actin, beta like 2 Kit ELISA, actin, cytoplasmic 1 Kit ELISA, ACTB Kit ELISA, Actb Kit ELISA, actb Kit ELISA, LOC100174883 Kit ELISA, LOC100304816 Kit ELISA, Act5C Kit ELISA, ACTG1 Kit ELISA, actb1 Kit ELISA, actb.L Kit ELISA, LOC100136352 Kit ELISA, POTEF Kit ELISA, ACTBL2 Kit ELISA, LOC109073280 Kit ELISA

Sujet:

Abbreviation: ACTB

Alias: PS1TP5BP1, PS1TP5-binding protein 1|actin, cytoplasmic 1|beta actin|β actin|beta cytoskeletal actin|βcytoskeletal actin

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction