IGF2 Kit ELISA

N° du produit ABIN6975915

Détail du produit IGF2 Kit ELISA

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: For the quantitative determination of human insulin-like growth factors 2 (IGF-2) concentrations in serum, plasma, cell culture supernates, urine, tissue homogenates.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of human IGF-2. No significant cross-reactivity or interference between human IGF-2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between human IGF-2 and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 15.6 pg/mL

Ingrédients:

• Assay plate
• Standard
• HRP-avidin (100 x concentrate)
• Biotin-antibody (100 x concentrate)
• Sample Diluent
• HRP-avidin Diluent
• Biotin-antibody Diluent
• Wash Buffer (25 x concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole:

1. Prepare reagents, samples and standards as instructed.
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C.
3. Remove the liquid of each well, don't wash.
4. Add 100 µL Biotin-antibody (1x) to each well. Incubate 1 hour at 37 °C.
5. Aspirate and wash 3 times.
6. Add 100 µL HRP-avidin (1x) to each well. Incubate 1 hour at 37 °C
7. Aspirate and wash 5 times.
8. Add 90 μL of TMB Substrate to each well. Incubate for 15-30 minutes at 37 °C. Protect from light.
9. Add 50 µL Stop Solution to each well. Read at 450 nm within 5 minutes.

Préparation des réactifs:

1. Biotin-antibody (1x) - Centrifuge the vial before opening. Biotin-antibody requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of Biotin-antibody + 990 μL of Biotin-antibody Diluent.
2. HRP-avidin (1x) - Centrifuge the vial before opening. HRP-avidin requires a 100-fold dilution. A suggested 100-fold dilution is 10 μL of HRP-avidin + 990 μL of HRP-avidin Diluent.
3. Wash Buffer (1x) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate (25 x) into deionized or distilled water to prepare 500 mL of Wash Buffer (1 x).
4. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 mL of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution of 4000 pg/mL. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions. Pipette 250 μL of Sample Diluent into each tube (S0-S6). Use the stock solution to produce a 2-fold dilution series (below). Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard (4000 pg/mL). Sample Diluent serves as the zero standard (0 pg/mL).

Note:

• Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
• Bring all reagents to room temperature (18-25 °C) before use for 30 min.
• Prepare fresh standard for each assay. Use within 4 hours and discard after use.
• Making serial dilution in the wells directly is not permitted.
• Please carefully reconstitute Standards according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10 μL for once pipetting.
• Distilled water is recommended to be used to make the preparation for reagents. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Note:

Recommend to dilute the serum or plasma samples with Sample Diluent (1:1000) before test. The suggested 1000-fold dilution can be achieved by adding 5 μL sample to 95 μL of Sample Diluent first, then complete the 1000-fold dilution by adding 5 μL of this solution to 245 μL of Sample Diluent. The recommended dilution factor is for reference only. The optimal dilution factor should be determined by users according to their particular experiments.

Précision du teste: Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C. If Biotin-antibody don't make recent use, better keep it store at HRP-avidin -20°C. Biotin-antibody Diluent Opened kit HRP-avidin Diluent Sample May be stored for up to 1 month at 2 - 8°C. Diluent Wash Buffer TMB Substrate Stop Solution *Provided this is within the expiration date of the kit.

Date de péremption: 6 months

Références pour IGF2 Kit ELISA (ABIN6975915)

Dai, Ji, Wright, Minichiello, Sadreyev, Avruch: "IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1." dans: eLife, Vol. 6, (2018) (PubMed).

Gao, Gu, Li, Cai, Peng, Tu, Kondo, Shinjo, Zhu, Zhang, Sekido, Han, Qian, Miao: "miR-615-5p is epigenetically inactivated and functions as a tumor suppressor in pancreatic ductal adenocarcinoma." dans: Oncogene, Vol. 34, Issue 13, pp. 1629-40, (2015) (PubMed).

Patel, Ellis, Howard, Boufraqech, Gara, Zhang, Quezado, Nilubol, Kebebew: "Analysis of IGF and IGFBP as prognostic serum biomarkers for adrenocortical carcinoma." dans: Annals of surgical oncology, Vol. 21, Issue 11, pp. 3541-7, (2015) (PubMed).

Yao, Yao, Wang, Dong, Wu, Qiu, Yan, Yu, Chen, Sai, Zhang, Yang: "Inhibition of autocrine IGF-II on effect of human HepG2 cell proliferation and angiogenesis factor expression." dans: Tumour biology, Vol. 33, Issue 5, pp. 1767-76, (2012) (PubMed).

Qian, Yao, Dong, Wu, Qiu, Yao, Li, Bian, Wang, Shi: "Characteristics of hepatic igf-ii expression and monitored levels of circulating igf-ii mRNA in metastasis of hepatocellular carcinoma." dans: American journal of clinical pathology, Vol. 134, Issue 5, pp. 799-806, (2010) (PubMed).

Détail du antigène

Antigène: IGF2 (Insulin-Like Growth Factor 2 (IGF2))

Autre désignation: insulin-like growth factor 2 (somatomedin A) (IGF2 Produits)

Synonymes: C11orf43 Kit ELISA, IGF-II Kit ELISA, PP9974 Kit ELISA, LOC100136550 Kit ELISA, igf2 Kit ELISA, igf2b Kit ELISA, wu:fc26e03 Kit ELISA, zgc:91781 Kit ELISA, igf-2 Kit ELISA, insigf Kit ELISA, pp9974 Kit ELISA, AL033362 Kit ELISA, Igf-2 Kit ELISA, Igf-II Kit ELISA, M6pr Kit ELISA, Mpr Kit ELISA, Peg2 Kit ELISA, IGFII Kit ELISA, RNIGF2 Kit ELISA, IGF-II-B Kit ELISA, igf2-a Kit ELISA, igf2-b Kit ELISA, insulin like growth factor 2 Kit ELISA, insulin-like growth factor II Kit ELISA, insulin-like growth factor 2a Kit ELISA, insulin like growth factor 2 S homeolog Kit ELISA, insulin-like growth factor 2 Kit ELISA, insulin-like growth factor 2 (somatomedin A) Kit ELISA, insulin like growth factor 2 L homeolog Kit ELISA, IGF2 Kit ELISA, LOC100136550 Kit ELISA, igf2a Kit ELISA, igf2.S Kit ELISA, Igf2 Kit ELISA, igf2.L Kit ELISA

Sujet:

Abbreviation: IGF2

Alias: C11orf43, FLJ22066, FLJ44734, INSIGF, pp9974, OTTHUMP00000011018|OTTHUMP00000011157|insulin-like growth factor 2|insulin-like growth factor II|insulin-like growth factor type 2|putative insulin-like

UniProt: P01344

Pathways: Hormone Activity, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Activated T Cell Proliferation