Glutamate Receptor 1 Kit ELISA

N° du produit ABIN6999489

Détail du produit Glutamate Receptor 1 Kit ELISA

Antigène: Glutamate Receptor 1 (GLUR1)

Reactivité: Lapin

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: The kit is a high sensitivity sandwich enzyme immunoassay for in vitro quantitative measurement in various sample types.

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Glutamate Receptor, Ionotropic, AMPA 1.

Sensibilité: 11.3 pg/mL

Classe de qualité: High Sensitivity

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 100 µL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
4. Aspirate and wash 3 times,
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
6. Aspirate and wash 5 times,
7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
8. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit is not used up all at once, remove only the strips and reagents for the current experiment and leave the remaining strips and reagents in the desired condition.
2. Standard - Reconstitute the standard with the Standard Diluent, keep it at room temperature for 10 minutes and shake it gently (do not let it foam). Please prepare tubes with Standard Diluent and make a dilution series. Mix each tube thoroughly before the next transfer. The last tube with Standard Diluent is the blank as 0 mg/mL.
3. Detection Reagent A and Detection Reagent B - Spin or centrifuge the stock of Detection Reagent A and B briefly before use. Dilute to working concentration (1:100) with Assay Diluent A or B, respectively.
4. Wash Solution - Dilute 20 mL of Wash Solution Concentrate (30x) with 580 mL of deionized or distilled water to make 600 mL of Wash Solution (1x).
5. TMB Substrate - Aspirate the required amount of solution with sterile tip and do not return the residual solution back into the vial.

Note:

1. Serial dilution directly in the wells is not recommended.
2. Prepare standard within 15 minutes before assay. Do not dissolve the reagents directly at 37 °C.
3. Detection Reagent A and B are sticky solutions, so pipette them slowly to reduce volume errors.
4. Reconstitute Standard or working solutions of Detection Reagent A and B carefully according to instructions, avoiding foaming and mixing gently until crystals are completely dissolved. To minimize inaccuracy caused by pipetting, use small volumes and ensure pipettes are calibrated. It is recommended to aspirate more than 10 µL for one-time pipetting.
5. The reconstituted Standard, Detection Reagent A and B can only be used once.
6. When crystals have formed in the Wash Solution concentrate (30x), warm it to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or preparation containers affect the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 ° C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

Date de péremption: 6 months

Détail du antigène

Antigène: Glutamate Receptor 1 (GLUR1)

Autre désignation: Glutamate Receptor, Ionotropic, AMPA 1 (GLUR1 Produits)

Synonymes: GLUH1 Kit ELISA, GLUR1 Kit ELISA, GLURA Kit ELISA, GluA1 Kit ELISA, HBGR1 Kit ELISA, gluR-A Kit ELISA, 2900051M01Rik Kit ELISA, Glr-1 Kit ELISA, Glr1 Kit ELISA, GluR-A Kit ELISA, GluRA Kit ELISA, Glur-1 Kit ELISA, Glur1 Kit ELISA, HIPA1 Kit ELISA, gluR-K1 Kit ELISA, GRIA1 Kit ELISA, glur1 Kit ELISA, Gria1 Kit ELISA, glur1b Kit ELISA, gria1.2 Kit ELISA, sb:eu293 Kit ELISA, ZfGluR1a Kit ELISA, glur1a Kit ELISA, gria1.1 Kit ELISA, zfGRIA1[a] Kit ELISA, glutamate ionotropic receptor AMPA type subunit 1 Kit ELISA, glutamate receptor, ionotropic, AMPA1 (alpha 1) Kit ELISA, glutamate receptor, ionotropic, AMPA 1 Kit ELISA, glutamate receptor, ionotropic, AMPA 1 L homeolog Kit ELISA, GluR1 protein Kit ELISA, Glutamate receptor 1 Kit ELISA, glutamate receptor, ionotropic, AMPA 1b Kit ELISA, glutamate receptor, ionotropic, AMPA 1a Kit ELISA, GRIA1 Kit ELISA, Gria1 Kit ELISA, gria1 Kit ELISA, gria1.L Kit ELISA, glur1 Kit ELISA, glr-1 Kit ELISA, gria1b Kit ELISA, gria1a Kit ELISA

Sujet: GLUH1, GLUR1, GLURA, HBGR1, GluR-K1, AMPA-selective glutamate receptor 1

Pathways: Signalisation PI3K-Akt