IL-15 Kit ELISA

N° du produit ABIN7014030

Détail du produit IL-15 Kit ELISA

Antigène: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31 pg/mL - 20 pg/mL

Seuil minimal de détection: 0.31 pg/mL

Application: ELISA

Fonction: For quantitative detection of IL-15 in serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of IL -15. No significant cross-reactivity or interference between IL-15 and analogues were observed.

Sensibilité: 0.875 pg/mL

Classe de qualité: High Sensitivity

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Plate sealer for 96 wells
• Standard
• Sample/Standard Dilution Buffer
• Assay Diluent
• Biotin-labeled Antibody (Concentrated)
• HRP-Streptavidin (HRP-SA)
• Biotin System (BS)
• BS Dilution Buffer
• TMB Substrate
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Volume d'échantillon: 50 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 50μL Assay Diluent to each well
3. Add 50μL standard or sample to each well. Incubate 90 minutes at 37 °C,
4. Aspirate and wash 2 times,
5. Add 100μL Biotin-labeled antibody to each well. Incubate 1 hour at 37 °C,
6. Aspirate and wash 2 times,
7. Add 100μL BS Working Solution to each well. Incubate 15 minutes at RT,
8. Aspirate and wash 3 times,
9. Add 100μL HRP-SA to each well. Incubate 30 minutes at 37 °C,
10. Aspirate and wash 3 times,
11. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
12. Add 50μL Stop Solution. Read at 450nm immediately.

Préparation des réactifs:

Bring all reagents and samples to room temperature for 20 minutes before use.

1. Wash Buffer: Dilute 30 mL Concentrated Wash Buffer to 750 mL Wash Buffer with deionized or distilled water. Put unused solution back at 2-8 °C.
   Note: If crystals have formed in the concentrate, you can warm it with 40 °C water bath (Heating temperature should not exceed 50 °C) and mix it gently until the crystals have completely been dissolved. The solution should be cooled to room temperature before use.
2. Standards:
   a) Add 1 mL Sample Dilution Buffer into one Standard tube (labeled as zero tube), keep the tube at room temperature for 10 minutes and mix them thoroughly.
   Note: If the standard tube concentration higher than the range of the kit, please dilute it and label as zero tube.
   b) Label 7 EP tubes with 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and blank respectively. Add 0.3 mL of the Sample Dilution Buffer into each tube. Add 0.3 mL of the above Standard solution (from zero tube) into 1st tube and mix them thoroughly. Transfer 0.3 mL from 1st tube to 2nd tube and mix them thoroughly, and so on. Sample Dilution Buffer is used for the blank control.
   Note: It is best to use Standard Solutions within 2 hours.
3. BS Working Solution: Prepare it within 15 minutes before experiment.
   a) Calculate required total volume of the working solution: 0.1 mL/well x quantity of wells. (Allow 0.1-0.2 mL more than the total volume.)
   b)Dilute the BS with BS Dilution Buffer at 1:100 and mix them thoroughly. (i.e. Add 1 μL of BS into 99 μL of BS Dilution Buffer.)
   Note: If crystals have formed in the BS, you can warm it with water (temperature should not exceed 30 °C) and mix it gently until the crystals have completely been dissolved.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).

Précision du teste: Intra-Assay: CV<8% Inter-Assay: CV<10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

Date de péremption: 6 months

Détail du antigène

Antigène: IL-15 (IL15) (Interleukin 15 (IL15))

Autre désignation: Interleukin 15 (IL15 Produits)

Synonymes: IL15 Kit ELISA, AI503618 Kit ELISA, IL-15 Kit ELISA, interleukin 15 Kit ELISA, IL15 Kit ELISA, Il15 Kit ELISA

Sujet: IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL -15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.

Pathways: Signalistation JAK/STAT, Glycosaminoglycan Metabolic Process