GDNF Kit ELISA

N° du produit ABIN921058

Détail du produit GDNF Kit ELISA

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Épitope: AA 109-211

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human GDNF

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: R109-I211

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <4pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: R109-I211

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the TGF-beta family. GDNF subfamily.

Tissue Specificity: In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. .

Plaque: Pre-coated

Protocole: human GDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GDNF has been precoated onto 96-well plates. Standards(NSO, R109-I211) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GDNF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human GDNF amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human GDNF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human GDNF standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 157, Standard deviation: 6.44, CV(%): 4.1
• Sample 2: n=16, Mean(pg/ml): 634, Standard deviation: 36.14, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 1416, Standard deviation: 72.22, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 144, Standard deviation: 10.66, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 667, Standard deviation: 54.69, CV(%): 8.2
• Sample 3: n=24, Mean(pg/ml): 1424, Standard deviation: 106.8, CV(%): 7.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour GDNF Kit ELISA (ABIN921058)

Niu, Li, Wu, Wu, Yan, Shang, Bai, Li, Hua: "Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells." dans: Oncotarget, Vol. 7, Issue 47, pp. 77532-77542, (2018) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." dans: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Liu, Wang, Shao, Liu: "Genetically modified Schwann cells producing glial cell line-derived neurotrophic factor inhibit neuronal apoptosis in rat spinal cord injury." dans: Molecular medicine reports, Vol. 9, Issue 4, pp. 1305-12, (2014) (PubMed).

Banerjee, Nürnberger, Hennerbichler, Riedl, Schuh, Hacobian, Teuschl, Eibl, Redl, Wolbank: "In toto differentiation of human amniotic membrane towards the Schwann cell lineage." dans: Cell and tissue banking, Vol. 15, Issue 2, pp. 227-39, (2014) (PubMed).

Chai, Guo, Li, Wang, Wang, Shi, Hu, Liu, Adah: "Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes." dans: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 100-7, (2013) (PubMed).

Wang, Hou, Yuan, Hou, Liu, Li, Zhang: "Association study between plasma GDNF and cognitive function in late-onset depression." dans: Journal of affective disorders, Vol. 132, Issue 3, pp. 418-21, (2011) (PubMed).

Yang, Zhou, Gao, Chen, Tu, Sun, Liu, He, Liu, Yuan: "Neuroprotective effects of bone marrow stem cells overexpressing glial cell line-derived neurotrophic factor on rats with intracerebral hemorrhage and neurons exposed to hypoxia/reoxygenation." dans: Neurosurgery, Vol. 68, Issue 3, pp. 691-704, (2011) (PubMed).

Détail du antigène

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Autre désignation: GDNF (GDNF Produits)

Synonymes: gdnf Kit ELISA, GDNF Kit ELISA, AI385739 Kit ELISA, gdnfa Kit ELISA, hfb1-gdnf Kit ELISA, ATF1 Kit ELISA, ATF2 Kit ELISA, HFB1-GDNF Kit ELISA, HSCR3 Kit ELISA, glial cell derived neurotrophic factor Kit ELISA, glial cell derived neurotrophic factor a Kit ELISA, glial cell line derived neurotrophic factor Kit ELISA, glial cell derived neurotrophic factor L homeolog Kit ELISA, Gdnf Kit ELISA, gdnfa Kit ELISA, GDNF Kit ELISA, gdnf.L Kit ELISA

Sujet:

Protein Function: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. .

Background: Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.

Synonyms: Glial cell line-derived neurotrophic factor,hGDNF,Astrocyte-derived trophic factor,ATF,GDNF,

Full Gene Name: Glial cell line-derived neurotrophic factor

Cellular Localisation: Secreted.

ID gène: 2668

UniProt: P39905

Pathways: Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration