IL-7 Kit ELISA

N° du produit ABIN921099

Détail du produit IL-7 Kit ELISA

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Épitope: AA 26-154

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-7

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: E26-I154

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <1pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: E26-I154

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-7 has been precoated onto 96-well plates. Standards(E.coli, E26-I154) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-7 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse IL-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-7 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 47.8, Standard deviation: 2.49, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 376, Standard deviation: 13.16, CV(%): 3.5
• Sample 3: n=16, Mean(pg/ml): 755, Standard deviation: 35.49, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 52.5, Standard deviation: 3.31, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 388, Standard deviation: 32.59, CV(%): 8.4
• Sample 3: n=24, Mean(pg/ml): 746, Standard deviation: 40.3, CV(%): 5.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-7 Kit ELISA (ABIN921099)

Guida, Luongo, Aviello, Palazzo, De Chiaro, Gatta, Boccella, Marabese, Zjawiony, Capasso, Izzo, de Novellis, Maione: "Salvinorin A reduces mechanical allodynia and spinal neuronal hyperexcitability induced by peripheral formalin injection." dans: Molecular pain, Vol. 8, pp. 60, (2012) (PubMed).

Détail du antigène

Antigène: IL-7 (IL7) (Interleukin 7 (IL7))

Autre désignation: IL7 (IL7 Produits)

Synonymes: IL7 Kit ELISA, IL-7 Kit ELISA, A630026I06Rik Kit ELISA, Il-7 Kit ELISA, hlb368 Kit ELISA, interleukin 7 Kit ELISA, IL7 Kit ELISA, Il7 Kit ELISA

Sujet:

Protein Function: Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

Background: Interleukin 7(IL7) is a protein that is encoded by the IL7 gene in humans. The gene for human interleukin 7(IL7) maps to chromosome 8 by Southern analysis of a somatic cell hybrid panel and to 8q12-q13 by in situ hybridization.1IL-7 is essential for survival and expansion of pathogenic T helper type 17(T(H)17) cells in experimental autoimmune encephalomyelitis(EAE).2

Synonyms: Interleukin-7,IL-7,Il7,Il-7,

Full Gene Name: Interleukin-7

Cellular Localisation: Secreted.

ID gène: 16196

UniProt: P10168

Pathways: Signalistation JAK/STAT