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IL23A Kit ELISA

IL23A Reactivité: Souris AA 23-335 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
N° du produit ABIN921111
  • Antigène Voir toutes IL23A Kits ELISA
    IL23A (Interleukin 23, alpha subunit p19 (IL23A))
    Épitope
    AA 20-196, AA 23-335
    Reactivité
    • 4
    • 3
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-23
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: (M23-S335)+(L20-A196)
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <2pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: (M23-S335)+(L20-A196)
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Secreted by activated dendritic cells (at protein level). Detected in various tissues with higher expression in polarized Th1 cells and activated macrophages. .

    Plaque
    Pre-coated
    Protocole
    mouse IL-23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-23 has been precoated onto 96-well plates. Standards(sf21, (M23-S335)+(L20-A196)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-23 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-23 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatant, serum or plasma (heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-23 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 255, Standard deviation: 11.73, CV(%): 4.6
    • Sample 2: n=16, Mean(pg/ml): 717, Standard deviation: 40.87, CV(%): 5.7
    • Sample 3: n=16, Mean(pg/ml): 1147, Standard deviation: 68.82, CV(%): 6,
    • Sample 1: n=24, Mean(pg/ml): 325, Standard deviation: 18.85, CV(%): 5.8
    • Sample 2: n=24, Mean(pg/ml): 836, Standard deviation: 53.5, CV(%): 6.4
    • Sample 3: n=24, Mean(pg/ml): 1327, Standard deviation: 95.5, CV(%): 7.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Zhang, Ding, Zhao, Yang, Zhang, Zhang, Cui: "Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats." dans: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1821-8, (2009) (PubMed).

  • Antigène Voir toutes IL23A Kits ELISA
    IL23A (Interleukin 23, alpha subunit p19 (IL23A))
    Autre désignation
    IL23A (IL23A Produits)
    Synonymes
    IL-23 Kit ELISA, IL-23A Kit ELISA, IL23P19 Kit ELISA, P19 Kit ELISA, SGRF Kit ELISA, FIL1 Kit ELISA, FIL1(ZETA) Kit ELISA, FIL1Z Kit ELISA, IL-1F7 Kit ELISA, IL-1H Kit ELISA, IL-1H4 Kit ELISA, IL-1RP1 Kit ELISA, IL-37 Kit ELISA, IL1F7 Kit ELISA, IL1H4 Kit ELISA, IL1RP1 Kit ELISA, p19 Kit ELISA, il23p19 Kit ELISA, IL23 Kit ELISA, interleukin 23 subunit alpha Kit ELISA, interleukin 37 Kit ELISA, interleukin 23, alpha subunit p19 Kit ELISA, IL23A Kit ELISA, IL37 Kit ELISA, Il23a Kit ELISA
    Sujet

    Protein Function: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. .

    Background: Interleukin-23 subunit alpha(IL-23) is a protein that in humans is encoded by the IL23A gene. IL-23 is a heterodimeric cytokine consisting of two subunits, one called p40, which is shared with another cytokine, IL-12, and another called p19(the IL-23 alpha subunit). IL-23 is an important part of the inflammatory response against infection. It promotes upregulation of the matrix metalloprotease MMP9, increases angiogenesis and reduces CD8+ T-cell infiltration. Recently, IL-23 has been implicated in the development of cancerous tumors. he International Radiation Hybrid Mapping Consortium mapped the p19 gene to chromosome 12(stSG47812).

    Synonyms: Interleukin-23 subunit alpha,IL-23 subunit alpha,IL-23-A,Interleukin-23 subunit p19,IL-23p19,Il23a,

    Full Gene Name: Interleukin-23 subunit alpha

    Cellular Localisation: Secreted . Secreted upon association with IL12B.
    ID gène
    83430
    UniProt
    Q9EQ14
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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