MYH11 Kit ELISA

N° du produit ABIN930691

Détail du produit MYH11 Kit ELISA

Antigène: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Attributs du produit: ELISA kit for the detection of Sm1 in the research laboratory
Alternative Names: Smith antigen ELISA kit

Information d'application

Indications d'application: Optimal conditions to be determined by end-user

Plaque: Pre-coated

Procédure de l'essai:

Highly purified RNP/Sm is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C.

Détail du antigène

Antigène: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Autre désignation: Sm1 (MYH11 Produits)

Sujet: Today the best investigated immunoreactive antigens are double stranded DNA (dsDNA), single stranded DNA, snRNP (small nuclear ribonucleoprotein particles), the complex RNP/Sm which is stabilized by ribonuleic acid as well as SSA (Ro) and SSB (La). Smith Antigen (Sm) is an autoantigen on a series of particles composed of RNA and protein. Unlike RNP, the sm antigen is resistant to RNase, but partially sensitive to trypsin. It is involved in the pathogenesis of Systemic lupus erythematosus (SLE).
Synonyms: Smith antigen ELISA kit.