Elastase Kit ELISA

N° du produit ABIN930710

Détail du produit Elastase Kit ELISA

Antigène: Elastase

Reactivité: Mammifères

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Analytical Method: Quantitative

Attributs du produit: ELISA kit for the detection of Elastase in the research laboratory
Alternative Names: Elastase ELISA kit

Information d'application

Indications d'application: Optimal conditions to be determined by end-user

Plaque: Pre-coated

Procédure de l'essai:

Highly purified Elastase is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C.

Détail du antigène

Antigène: Elastase

Abstract: Elastase Produits

Sujet: Elastase is a serine protease with a sequence homology of 54 % to that of proteinase 3. It occurs mainly in polymorphnuclear neutrophilic granulocytes (PMN), in macrophages and endothelial cells. The dismantling of proteoglycans by neutrophiles is mainly due to elastase' proteolytic activity. Furthermore, elastase participates decisively in tissue destruction connected with emphysemas and rheumatoid arthritis. Autoantibodies against this antigen are generally associated with inflammatory rheumatic disorders, e.g. rheumatoid arthritis and vasculitis.
Synonyms: Elastase ELISA kit.