GAPDH Kit ELISA
-
- Antigène Voir toutes GAPDH Kits ELISA
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156-10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Fonction
- The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of human GAPDH in tissue homogenates and other biological fluids.
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of human GAPDH. No significant cross-reactivity or interference between human GAPDH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us - complete the cross-reactivity detection between human GAPDH and all the analogues, therefore, cross reaction may still exist.
- Sensibilité
-
The minimum detectable dose of human GAPDH is typically less than 0.051 ng/mL.
The Sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero calibrator plus three standard deviations. - Attributs du produit
- The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to GAPDH. Calibrators or samples are then added to the appropriate microtiter plate wells with a biotin- conjugated polyclonal antibody preparation specific for GAPDH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GAPDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 10 nm. The concentration of GAPDH in the samples is then determined by comparing the O.D. of the samples to the calibration curve.
- Ingrédients
-
Pre-coated, ready to use 96-well strip plate (1x)
Calibrator (lyophilized) (2x)
Calibrator Diluent (1 x 20 mL)
Detection Reagent A (1 x 120 µL)
Detection Reagent B (1 x 120 µL)
Assay Diluent A (2X concentrate) (1 x 6 mL)
Assay Diluent B (2X concentrate) (1 x 6 mL)
TMB Substrate (1 x 9 mL)
Stop Solution (1 x 6 mL)
Wash Buffer (30X concentrate) (1 x 20 mL)
Plate sealer for 96 wells (4x). - Matériel non inclus
-
1. Microplate reader with 450 +/- 10 nm filter.
2. Precision single and multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. De-ionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
- Discover our best selling GAPDH Kit ELISA
- Top Product
- Discover our top product GAPDH Kit ELISA
-
-
- Commentaires
-
The calibration curve concentrations used for the ELISA's were 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.312 ng/mL, 0.156 ng/mL.
- Plaque
- Pre-coated
- Calcul des résultats
-
Average the duplicate readings for each calibrator, control, and samples and subtract the average zero calibrator optical density. Create a calibration curve on log-log graph paper, with GAPDH concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the calibrator points and it can be determined by regression analysis. Using some plot software is also recommended. If samples have been diluted, the concentration read from the calibration curve must be multiplied by the dilution factor.
- Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- All the reagents should be kept according to the labels on vials. The Calibrator, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20° C upon being received. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. Opened test kits will remain stable until the expiration date shown, provided it is stored as prescribed above.
- Date de péremption
- The expiry date is stated on the label.
-
- Antigène Voir toutes GAPDH Kits ELISA
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Autre désignation
- GAPDH (GAPDH Produits)
- Synonymes
- G3PD Kit ELISA, GAPD Kit ELISA, Gapd Kit ELISA, BEST:GH12586 Kit ELISA, CG12055 Kit ELISA, Dmel\\CG12055 Kit ELISA, GA3PDH Kit ELISA, GADPH Kit ELISA, GAP Kit ELISA, GAPDH Kit ELISA, GAPDH I Kit ELISA, GAPDH-1 Kit ELISA, GAPDH1 Kit ELISA, GAPDHI Kit ELISA, Gapdh Kit ELISA, Gapdh-1 Kit ELISA, Gapdh43E Kit ELISA, gadph Kit ELISA, gapdh Kit ELISA, gapdh-1 Kit ELISA, gh12586 Kit ELISA, cb609 Kit ELISA, gapd Kit ELISA, mg:bb02e05 Kit ELISA, wu:fb33a10 Kit ELISA, wu:ft80f05 Kit ELISA, KNC-NDS6 Kit ELISA, g3pd Kit ELISA, G3PDH Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase Kit ELISA, Glyceraldehyde 3 phosphate dehydrogenase 1 Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase S homeolog Kit ELISA, glyceraldehyde-3-phosphate dehydrogenase, type I Kit ELISA, olfactory receptor 8K3 Kit ELISA, GAPDH Kit ELISA, Gapdh Kit ELISA, Gapdh1 Kit ELISA, gapdh Kit ELISA, gapdh.S Kit ELISA, gapDH Kit ELISA, LOC100404960 Kit ELISA, LOC614985 Kit ELISA
-