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Cell ELISA Kits

"Cell ELISA", également connu sous le nom de In-Cell ELISA, Cell-based ELISA ou In-Cell Western, est une méthode d'immunocytochimie quantitative permettant de mesurer les niveaux de protéines ou les modifications post-traductionnelles des cellules cultivées. Il s'agit d'une méthode simple et sensible pour la quantification des protéines intracellulaires dans les cellules entières. Les ELISA cellulaires sont parfaitement adaptés pour surveiller les effets de la stimulation et/ou de l'inhibition de traitements, de composés (par exemple, siRNA, produits chimiques) sur des molécules cibles.

La méthode Cell-ELISA présente quelques avantages clés. La mesure de la cible antigénique dans des cellules vivantes en culture annule toute interférence ou effet confondant résultant de l'utilisation de réactifs de lyse agressifs. En outre, la méthode en cellule est plus rapide, plus évolutive et plus performante que d'autres méthodes protéomiques comparables comme le Western Blotting et/ou l'ELISA en sandwich. L'ELISA cellulaire est pratique et efficace pour une utilisation quotidienne. Les kits ELISA cellulaires nécessitent des cellules vivantes et adhérentes pour la mesure, et ne sont donc pas adaptés à la mesure de types d'échantillons standard comme le plasma, le sérum ou les milieux de culture tissulaire. Les chercheurs qui souhaitent analyser ces types d'échantillons devraient plutôt choisir un ELISA sandwich traditionnel ou un ELISA de format compétitif.

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1.469 Résultats :

ERK1/2 Kit ELISA

MAPK1/3 Reactivité: Humain, Rat, Souris pThr202 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentrations of EGF for 10 min at 37

5 images

10 références

N° du produit ABIN1981830

Fiche technique

Fiche de données

JNK Kit ELISA

MAPK8 Reactivité: Humain, Souris, Rat pThr183 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

Hela cells were stimulated by different concentrations of anisomycin for 15 minutes at 37 °C

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

6 images

4 références

N° du produit ABIN1981833

Fiche technique

Fiche de données

STAT1 Kit ELISA

STAT1 Reactivité: Humain, Souris, Rat pTyr701 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C.

Example of how to seed cells for cell-based assay

A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37 °C

6 images

2 références

N° du produit ABIN1981837

Fiche technique

Fiche de données

STAT3 Kit ELISA

STAT3 Reactivité: Humain, Rat, Souris pTyr705 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431cells.

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration EGF for 30 min at 37 °C

6 images

3 références

N° du produit ABIN1981839

Fiche technique

Fiche de données

STAT5A Kit ELISA

STAT5A Reactivité: Humain, Rat, Souris pTyr694 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentrations of EGF for 10 minutes at 37 °C

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C

6 images

1 reference

N° du produit ABIN1981843

Fiche technique

Fiche de données

MAPK14 Kit ELISA

MAPK14 Reactivité: Humain, Rat, Souris pThr180 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

This picture shows the reagent preparation.

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

Cell-Based protein phosphorylation procedure

Hela cells were stimulated by different concentrations of anisomycin for 15 minutes at 37 °C

Example of how to seed cells for cell-based assay

6 images

4 références

N° du produit ABIN1981835

Fiche technique

Fiche de données

STAT1,STAT3,STAT5 Kit ELISA

Reactivité: Humain, Souris, Rat pTyr705 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431cells.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration EGF for 30 min at 37 °C

This picture shows the reagent preparation.

A431 cells were stimulated by different concentrations of EGF for 30 minutes at 37 °C.

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

A431 cells were stimulated by different concentrat ions of EGF for 30 minutes at 37 °C

7 images

4 références

N° du produit ABIN1981847

Fiche technique

Fiche de données

ERK1/2, JNK, p38 MAPK Kit ELISA

Reactivité: Humain, Souris, Rat pThr183 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

Cell-Based protein phosphorylation procedure

Hela cells were stimulated by different concentrations of anisomycin for 1 hour at 37 °C

A431 cells were stimulated by different concentrations of EGF for 10 min at 37 °C.

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

Western blot analysis of extracts from 1 μg/mL Anisomycin treated Hela cells.

This picture shows the reagent preparation.

9 images

6 références

N° du produit ABIN1981832

Fiche technique

Fiche de données

STAT6 Kit ELISA

STAT6 Reactivité: Humain, Rat, Souris pTyr641 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

Cell-Based protein phosphorylation procedure

A431 cells were stimulated by different concentration of recombinant human EGF for 10 min at 37 °C

5 images

1 reference

N° du produit ABIN1981845

Fiche technique

Fiche de données

STAT4 Kit ELISA

STAT4 Reactivité: Humain, Souris, Rat pTyr693 Colorimetric Cell ELISA Cell Culture Cells

Western blot analysis of extracts from 100 ng/mL hEGF treated A431 cells.

Cell-Based protein phosphorylation procedure

Image no. 3 for Signal Transducer and Activator of Transcription 4 (STAT4) ELISA Kit (ABIN1981841)

Example of how to seed cells for cell-based assay

This picture shows the reagent preparation.

5 images

3 références

N° du produit ABIN1981841

Fiche technique

Fiche de données

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