Expected / apparent Molecular Weight of the Antigene: 23 / 24 kDa for Arabidopsis thaliana
Purification
affinity purified
Immunogène
BSA-conjugated synthetic peptide derived from Arabidopsis thaliana Lhcb6, UniProt: Q9LMQ2, TAIR:At1g15820. This sequence is highly conserved in angiosperms (monocots and dicots) and gymnosperms.
Isotype
IgG
Indications d'application
Recommended Dilution: 1 : 2000, detected with standard ECL (WB).
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution please add 200 µL of sterile water
Buffer
PBS pH 7.4
Conseil sur la manipulation
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Stock
-20 °C
Ferroni, Suorsa, Aro, Baldisserotto, Pancaldi et al.: "Light acclimation in the lycophyte Selaginella martensii depends on changes in the amount of photosystems and on the flexibility of the light-harvesting complex II antenna association with both ..." dans: The New phytologist, (2016) (PubMed).
Wientjes, van Amerongen, Croce: "Quantum Yield of Charge Separation in Photosystem II: Functional Effect of Changes in the Antenna Size upon Light Acclimation." dans: The journal of physical chemistry. B, Vol. 117, Issue 38, pp. 11200-8, (2013) (PubMed).
Zienkiewicz, Ferenc, Wasilewska, Romanowska: "High light stimulates Deg1-dependent cleavage of the minor LHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana." dans: Planta, Vol. 235, Issue 2, pp. 279-88, (2012) (PubMed).
Antigène
Lhcb6
(Cp24 Chlorphyll A/B-Binding Protein of Plant Psii (Lhcb6))
Sujet
AGI Code: At1g15820 Lhcb6 is one of the 3 highly conserved minor chlorophyll a/b-binding proteins exclusively associated with Photosystem II in plants and algae. Together with Lhcb4 and Lhcb5, it regulates the energy flow from the outer antenna to the reaction center through the action of the xanthophyll cycle.
Poids moléculaire
expected: 23 kDa, apparent: 24 kDa for Arabidopsis thaliana