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MSP2N2 Protein (His tag)

MSP2N2 Origine: Humain Hôte: Escherichia coli (E. coli) Recombinant > 90 % STB
N° du produit ABIN3199238
  • Antigène
    MSP2N2 (Nanodisc Membrane Scaffold Protein 2N2 (MSP2N2))
    Type de proteíne
    Recombinant
    Origine
    Humain
    Source
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette MSP2N2 protéine est marqué à la His tag.
    Application
    Stabilization (STB)
    Fonction
    Forms nanodisc structures in combination with phospholipids
    Specificité
    Stabilization of membrane proteins
    Attributs du produit
    • MSP2N2 protein, human sequence, corresponds to duplicated MSP1D1 protein
    • N-terminal 6x His-tag
    • Resulting nanodisc diameter: ca. 15-17 nm

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Pureté
    > 90 %
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Ingrédients
    Lyophilized MSP protein
    Matériel non inclus
    • 37 °C incubator
    • 4 °C incubator
    • End-over-end shaker
    • Micropipettor and Micropipetting tips
    • Gel filtration column
    • FPLC instrument with integrated UV detector and fraction collector
    • Magnetic stirrer
    • Centrifuge for 2 mL microtubes
    • 2 mL microtubes
    • Palmitoyl-oleoyl-phosphatidylcholine (POPC)
    • Dimyristoyl-glycero-phosphocholine (DMPC) or other suitable phospholipid or phospholipid mixture
    • Sodium cholate
    • Biobeads SM-2
    • Single use syringe
    • Single use needle
    • 0.45 micron filter
    • Protein concentrator
    • SDS PAGE reagents and equipment

    • Optional: Western Blot reagents and equipment and PentaHis Antibody
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Sample Volume for an assay: >1 mg purified membrane protein In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Protocole
    Stabilization of membrane proteins using nanodiscs
    Calcul des résultats

    SDS-PAGE / Western Blot

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Stock
    -20 °C
  • Antigène
    MSP2N2 (Nanodisc Membrane Scaffold Protein 2N2 (MSP2N2))
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