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Neuroligin 1/2/3/4 anticorps (AA 1-695)

NLGN1/2/3/4 Reactivité: Souris, Rat IP, ELISA, WB Hôte: Souris Monoclonal 4F9 unconjugated
N° du produit ABIN1742285
  • Antigène
    Neuroligin 1/2/3/4 (NLGN1/2/3/4)
    Épitope
    AA 1-695
    Reactivité
    Souris, Rat
    Hôte
    Souris
    Clonalité
    Monoclonal
    Application
    Immunoprecipitation (IP), ELISA, Western Blotting (WB)
    Specificité
    Recognizes rat and mouse neuroligins 1-3 and rat neuroligin 4.
    Purification
    purified IgG
    Immunogène
    Recombinant protein of rat neuroligin 1 containing the extracellular sequence (aa 1-695).
    Clone
    4F9
    Isotype
    IgG2a
  • Indications d'application
    WB: 1 : 1000 (AP staining)
    ICC: not recommended
    IHC: not recommended
    Commentaires

    ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
    Buffer
    PBS
    Conseil sur la manipulation
    Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
    Stock
    -20 °C
    Stockage commentaire
    Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
  • Jadhav, Katina, Kovac, Kazmerova, Novak, Zilka: "Truncated tau deregulates synaptic markers in rat model for human tauopathy." dans: Frontiers in cellular neuroscience, Vol. 9, pp. 24, (2015) (PubMed).

    Fujita-Jimbo, Tanabe, Yu, Kojima, Mori, Li, Iwamoto, Yamagata, Momoi, Momoi: "The association of GPR85 with PSD-95-neuroligin complex and autism spectrum disorder: a molecular analysis." dans: Molecular autism, Vol. 6, pp. 17, (2015) (PubMed).

    Vasileva, Horstmann, Geumann, Gitler, Kuner: "Synapsin-dependent reserve pool of synaptic vesicles supports replenishment of the readily releasable pool under intense synaptic transmission." dans: The European journal of neuroscience, Vol. 36, Issue 8, pp. 3005-20, (2012) (PubMed).

    Bottos, Destro, Rissone, Graziano, Cordara, Assenzio, Cera, Mascia, Bussolino, Arese: "The synaptic proteins neurexins and neuroligins are widely expressed in the vascular system and contribute to its functions." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 49, pp. 20782-7, (2010) (PubMed).

    De Jaco, Comoletti, Kovarik, Gaietta, Radic, Lockridge, Ellisman, Taylor: "A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family." dans: The Journal of biological chemistry, Vol. 281, Issue 14, pp. 9667-76, (2006) (PubMed).

    Chih, Engelman, Scheiffele: "Control of excitatory and inhibitory synapse formation by neuroligins." dans: Science (New York, N.Y.), Vol. 307, Issue 5713, pp. 1324-8, (2005) (PubMed).

  • Antigène
    Neuroligin 1/2/3/4 (NLGN1/2/3/4)
    Autre désignation
    Neuroligin
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