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Nlrp9b Protein (AA 1-1003) (Strep Tag)

NLRP9B Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). ELISA, SDS, WB
N° du produit ABIN3135622
  • Antigène
    Nlrp9b (NLRP9B) (NLR Family, Pyrin Domain Containing 9B (NLRP9B))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1003
    Origine
    Souris
    Source
    Tobacco (Nicotiana tabacum)
    Purification/Conjugué
    Cette Nlrp9b protéine est marqué à la Strep Tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MAGSSGYGLL KLLQKLSDEE FQRFKELLRE EPEKFKLKPI SWTKIENSSK ESLVTLLNTH YPGQAWNMML SLFLQVNRED LSIMAQKKKR HKQTKYKKFM KTTFERIWTL ETNTHIPDRN YHLIVEVQYK ALQEIFDSES EPVTAIVAGT TGEGKTTFLR KAMLDWASGV LLQNRFQYVF FFSVFSLNNT TELSLAELIS STLPESSETV DDILSDPKRI LFILDGFDYL KFDLELRTNL CNDWRKRLPT QIVLSSLLQK IMLPGCSLLL ELGQISVPKI RHLLKYPRVI TMQGFSERSV EFYCMSFFDN QRGIEVAENL RNNEVLHLCS NPYLCWMFCS CLKWQFDREE EGYFKAKTDA AFFTNFMVSA FKSTYAHSPS KQNRARLKTL CTLAVEGMWK ELFVFDSEDL RRNGISESDK AVWLKMQFLQ THGNHTVFYH PTLQSYFAAM FYFLKQDKDI CVPVIGSIPQ LLGNMYARGQ TQWLQLGTFL FGLINEQVAA LLQPCFGFIQ PIYVRQEIIC YFKCLGQQEC NEKLERSQTL FSCLRDSQEE RFVRQVVDLL EEITVDISSS DVLSVTAYAL QKSSKLKKLH LHIQKTVFSE IYCPDHCKTR TSIGKRRNTA EYWKTLCGIF CNLYVLDLDS CQFNKRAIQD LCNSMSPTPT VPLTAFKLQS LSCSFMADFG DGSLFHTLLQ LPHLKYLNLY GTYLSMDVTE KLCAALRCSA CRVEELLLGK CGISSKACGI IAISLINSKV KHLSLVENPL KNKGVMSLCE MLKDPSCVLQ SLMLSYCCLT FIACGHLYEA LLSNKHLSLL DLGSNFLEDT GVNLLCEALK DPNCTLKELW LPGCFLTSQC CEEISAVLIC NRNLKTLKLG NNNIQDTGVR QLCEALSHPN CNLECLGLDL CEFTSDCCKD LALALTTCKT LNSLNLDWKT LDHSGLVVLC EALNHKRCNL KMLGLDKSAF SEESQTLLQD VEKKNNNLNI LHHPWFEAER NKRGTRLVWN SRN
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Attributs du produit
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Pureté
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Nlrp9b (NLRP9B) (NLR Family, Pyrin Domain Containing 9B (NLRP9B))
    Autre désignation
    Nlrp9b
    Synonymes
    Nalp-delta Protein, Nalp9b Protein, Nlrp9 Protein, NLR family, pyrin domain containing 9B Protein, Nlrp9b Protein
    Sujet
    NACHT, LRR and PYD domains-containing protein 9B (NALP-delta),FUNCTION: As the sensor component of the NLRP9 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens, including rotavirus, initiates the formation of the inflammasome polymeric complex, made of NLRP9, PYCARD and CASP1. Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and release in the extracellular milieu. The active cytokines stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. NLRP9 inflammasome activation may be initiated by DHX9 interaction with viral double-stranded RNA (dsRNA), preferentially to short dsRNA segments. {ECO:0000269|PubMed:28636595}.
    Poids moléculaire
    114.8 kDa
    UniProt
    Q66X22
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