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NSP1 anticorps

NSP1 Reactivité: Saccharomyces cerevisiae ICC, IF, IHC, WB Hôte: Souris Monoclonal 32D6 unconjugated
N° du produit ABIN1580452
  • Antigène
    NSP1 (Nsp1p (NSP1))
    Reactivité
    Saccharomyces cerevisiae
    Hôte
    Souris
    Clonalité
    Monoclonal
    Application
    Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
    Purification
    conc. tissue culture supernatant
    Stérilité
    Sterile filtered
    Clone
    32D6
    Isotype
    IgG3
  • Indications d'application
    For western blots of yeast protein samples, use MCA-32D6 diluted 1/10,000 (cell lysates) to 1/50,000 (nuclear fractions), followed by chemiluminescent detection (ECL). For other (non-ECL) western detection methods, try MCA-32D6 diluted 1/1,000 to 1/5,000. For immunofluorescence on yeast cells, use MCA-32D6 diluted 1/1,000 to 1/10,000. Antibody preparation contains 10mM sodium azide preservative .
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    It is supplied as a sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The antibody class is IgG1 and the antibody concentration is about 1mg/mL.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store at 4°C short term or -20°C long term.
  • Antigène
    NSP1 (Nsp1p (NSP1))
    Autre désignation
    Nsp1p
    Sujet
    MCA-32D6 recognizes Nsp1p, a nuclear pore complex protein, and this antibody is a useful marker of yeast nuclear pores. The monoclonal antibody MCA-32D6 was initially identified as directed against a nuclear pore complex antigen by immunofluorescence localization. A screen of a lgt11 expression library yielded a single positive clone carrying an insert encoding approx. 66% of the C-terminal portion of Nsp1p. To confirm that MCA-32D6 possessed high affinity for Nsp1p, strain RS453, which expresses a shortened isoform of Nsp1p, was compared to the wildtype strain BJ5465. NSP1 in the RS453 strain contains an internal deletion that removes the coding sequence for 6 FXFG repeats, which are not essential for protein function, and encodes a protein that has been observed to migrate at approximately 85 kDal on SDS-PAGE gels. The predicted size of wildtype Nsp1p is 86.5 kDal, but Nsp1p has been observed to migrate on SDS gels at approx. 100 kDal. In our SDS-PAGE gels, the apparent molecular mass of wild type Nsp1p was 108 kDal, whereas the isoform of Nsp1p expressed in the RS453 strain migrated at 91 kDal. The detection of two protein bands of apparent sizes 108 kDal and 91 kDal in the BJ5465 and RS453 strains, respectively, demonstrated that MCA-32D6 recognized the pore complex protein Nsp1p.
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