When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
ATG5
(ATG5 Autophagy Related 5 (ATG5))
Synonymes
APG5 Peptide, APG5-LIKE Peptide, APG5L Peptide, ASP Peptide, hAPG5 Peptide, ATG5 Peptide, CG1643 Peptide, DmAtg5 Peptide, Dmel\\CG1643 Peptide, atg5 Peptide, 2010107M05Rik Peptide, 3110067M24Rik Peptide, AW319544 Peptide, Apg5l Peptide, Atg5l Peptide, C88337 Peptide, Paddy Peptide, apg5l Peptide, zgc:100934 Peptide, ATATG5 Peptide, AUTOPHAGY 5 Peptide, MKP11.20 Peptide, MKP11_20 Peptide, autophagy related 5 Peptide, Autophagy-related 5 Peptide, ATG5 autophagy related 5 homolog (S. cerevisiae) Peptide, autophagy related 5 L homeolog Peptide, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 Peptide, autophagy protein Apg5 family Peptide, ATG5 Peptide, Atg5 Peptide, atg5 Peptide, atg5.L Peptide, APG5 Peptide