When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
STUB1
(STIP1 Homology and U-Box Containing Protein 1 (STUB1))
Synonymes
CHIP Peptide, HSPABP2 Peptide, NY-CO-7 Peptide, SDCCAG7 Peptide, UBOX1 Peptide, 0610033N24Rik Peptide, 2210017D18Rik Peptide, 2310040B03Rik Peptide, AW046544 Peptide, Chip Peptide, wu:fc22f04 Peptide, zgc:56076 Peptide, STUB1 Peptide, chip Peptide, ATCHIP Peptide, CARBOXYL TERMINUS OF HSC70-INTERACTING PROTEIN Peptide, carboxyl terminus of HSC70-interacting protein Peptide, STIP1 homology and U-box containing protein 1 Peptide, STIP1 homology and U-Box containing protein 1 Peptide, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase L homeolog Peptide, STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase Peptide, STIP1 homology and U box-containing protein 1 Peptide, carboxyl terminus of HSC70-interacting protein Peptide, STUB1 Peptide, Stub1 Peptide, stub1 Peptide, stub1.L Peptide, LOC100282870 Peptide, CHIP Peptide