When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
GRAP
(GRB2-Related Adaptor Protein (GRAP))
Synonymes
8430435N19Rik Peptide, BB233594 Peptide, GRAP Peptide, grap Peptide, zgc:110202 Peptide, MGC109892 Peptide, si:dkeyp-9d4.1 Peptide, Grap Peptide, GRB2-related adaptor protein Peptide, GRB2-related adapter protein Peptide, GRB2-related adaptor protein a Peptide, GRB2-related adaptor protein b Peptide, GRAP Peptide, Grap Peptide, LOC489534 Peptide, grapa Peptide, grapb Peptide, LOC100727005 Peptide