When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
alpha-Mannosidase II (MAN2A1)
(Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1))
Synonymes
CG18474 Peptide, CG18802 Peptide, CG8139 Peptide, DM-GII.1 Peptide, Dmel\\CG18802 Peptide, GMII Peptide, GmII Peptide, MAN-2 Peptide, Man Peptide, dGMII Peptide, dGMIIb Peptide, MAN2A1 Peptide, si:ch211-131c14.1 Peptide, Mana-2 Peptide, Mana2 Peptide, Map-2 Peptide, GOLIM7 Peptide, MANA2 Peptide, MANII Peptide, Manno Peptide, alpha-Mannosidase class II a Peptide, mannosidase alpha class 2A member 1 Peptide, mannosidase, alpha, class 2A, member 1 Peptide, mannosidase 2, alpha 1 Peptide, alpha-Man-IIa Peptide, MAN2A1 Peptide, man2a1 Peptide, Man2a1 Peptide