This peptide is the immunizing peptide for ABIN308401 and may be used for blocking experiments
Purification
Rabbit Anti-c-Jun (Phospho-Ser63) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
CDHR5
(Mucin and Cadherin-Like (CDHR5))
Synonymes
MU-PCDH Peptide, MUCDHL Peptide, MUPCDH Peptide, 1810074H01Rik Peptide, AI481143 Peptide, Mucdhl Peptide, Mupcdh Peptide, cadherin related family member 5 Peptide, cadherin-related family member 5 Peptide, CDHR5 Peptide, Cdhr5 Peptide