When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
AKR1B10
(Aldo-Keto Reductase Family 1, Member B10 (Aldose Reductase) (AKR1B10))
Synonymes
AKR1B11 Peptide, AKR1B12 Peptide, ALDRLn Peptide, ARL-1 Peptide, ARL1 Peptide, HIS Peptide, HSI Peptide, 2310005E10Rik Peptide, Akr1b16 Peptide, AKR Peptide, AKR1B10 Peptide, Akr1b10 Peptide, aldo-keto reductase family 1 member B10 Peptide, aldo-keto reductase family 1, member B10 (aldose reductase) Peptide, aldo-keto reductase family 1 member B10-like 2 Peptide, AKR1B10 Peptide, Akr1b10 Peptide, AKR1B10L2 Peptide, LOC100585902 Peptide, LOC100723118 Peptide, LOC101107697 Peptide