When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
AKR1B1
(Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))
Synonymes
ADR Peptide, ALDR1 Peptide, ALR2 Peptide, AR Peptide, ALDRED Peptide, ALR-P-I Peptide, Akr1b3 Peptide, Akr1b4 Peptide, Aldr1 Peptide, Alr Peptide, RATALDRED Peptide, akr1b7 Peptide, zgc:86611 Peptide, aldo-keto reductase family 1 member B Peptide, aldo-keto reductase family 1, member B1 (aldose reductase) Peptide, aldo-keto reductase family 1, member B1 (aldose reductase) S homeolog Peptide, AKR1B1 Peptide, Akr1b1 Peptide, akr1b1.S Peptide, akr1b1 Peptide