When peptides are reconstituted in 200ul water, the concentration would be also 0.5mg/ml. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20min. Then incubate and develop in parallel the two blots.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
Buffer
100µg of Lyophilised peptide, formulation De-ionized (DI) water.
Stock
-20 °C
Stockage commentaire
Shipped at ambient temperature, store at -20°C.
Antigène
TBC1D4
(TBC1 Domain Family, Member 4 (TBC1D4))
Synonymes
AS160 Peptide, 5930406J04Rik Peptide, A930035N22 Peptide, AV295684 Peptide, As160 Peptide, RGD1561609 Peptide, TBC1 domain family member 4 Peptide, TBC1 domain family, member 4 Peptide, TBC1D4 Peptide, Tbc1d4 Peptide