Purified recombinant Human Cyclophilin B protein Expression System: E.coli Bioactivity: Specific activity is > 170 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin. Molecular weight on SDS-PAGE will appear higher.
Pureté
> 95 % pure
niveau d'endotoxine
< 1.0 EU per µg of protein (determined by LAL method)
Cyclophilin B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Procédure de l'essai
Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA). 2. Add recombinant Cyclophilin B protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer. 3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer. 4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately. 5. Record the increase in A410 nm for 20 minutes
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Supplied as a liquid in 20 mM Tris, pH 8.0, containing 20 mM NaCl, 0.0 mM DTT, and 10 % glycerol.
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freeze/thaw cycles.
Stock
RT/-20 °C
Stockage commentaire
Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques. Alternative Names: Cphn 2 protein, AA408962 protein, CYP S1 protein, Cyclophilin B protein, PPIB protein, MGC14109 protein, S cyclophilin., 26- 216aa protein, PPIase protein, Cphn2 protein, CyP 20b protein, SCYLP protein, AA553318 protein, AI844835 protein, Peptidyl prolyl cis trans isomerase B precursor protein, Peptidylprolyl isomerase B protein, Rotamase protein, CYPB protein, MGC2224 protein, peptidyl prolyl cis trans isomerase B protein, CYP-S1: Cyclophilin-B protein