Potassium Channel KAT3 anticorps
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- Antigène
- Potassium Channel KAT3 (KAT3)
- Reactivité
- Arabidopsis thaliana
- Hôte
- Lapin
- Clonalité
- Polyclonal
- Application
- Western Blotting (WB)
- Réactivité croisée (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Homologie
- Arabidopsis thaliana
- Attributs du produit
- Expected / apparent Molecular Weight of the Antigene: 75.5 / 75 kDa
- Purification
- serum
- Immunogène
- KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
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- Indications d'application
- 1: 50 with 125I (WB)
- Commentaires
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In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 100 μL of sterile water.
- Conseil sur la manipulation
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Stock
- -20 °C
- Stockage commentaire
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Antigène
- Potassium Channel KAT3 (KAT3)
- Autre désignation
- KC1
- Synonymes
- anticorps A. thaliana low-K+-tolerant 1, anticorps ARABIDOPSIS THALIANA K+ RECTIFYING CHANNEL 1, anticorps ATKC1, anticorps AtLKT1, anticorps F4D11.150, anticorps F4D11_150, anticorps K+ INWARD RECTIFYING CHANNEL PROTEIN, anticorps KC1, anticorps potassium channel in Arabidopsis thaliana 3, anticorps potassium channel protein, anticorps KAT3
- Sujet
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AGI Code: At4g32650
KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC - Poids moléculaire
- expected: 75.5 kDa, apparent: 75 kDa
- UniProt
- P92960
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