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Potassium Channel KAT3 anticorps

KAT3 Reactivité: Arabidopsis thaliana WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN488529
  • Antigène
    Potassium Channel KAT3 (KAT3)
    Reactivité
    Arabidopsis thaliana
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Application
    Western Blotting (WB)
    Réactivité croisée (Details)
    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
    Homologie
    Arabidopsis thaliana
    Attributs du produit
    Expected / apparent Molecular Weight of the Antigene: 75.5 / 75 kDa
    Purification
    serum
    Immunogène
    KLH-conjugated peptide derived from Arabidopsis thaliana KC1 protein P92960, At4g32650
  • Indications d'application
    1: 50 with 125I (WB)
    Commentaires

    In the work of Honsbein et al. 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning. ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems. In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Conseil sur la manipulation
    Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Stock
    -20 °C
    Stockage commentaire
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Antigène
    Potassium Channel KAT3 (KAT3)
    Autre désignation
    KC1
    Synonymes
    anticorps A. thaliana low-K+-tolerant 1, anticorps ARABIDOPSIS THALIANA K+ RECTIFYING CHANNEL 1, anticorps ATKC1, anticorps AtLKT1, anticorps F4D11.150, anticorps F4D11_150, anticorps K+ INWARD RECTIFYING CHANNEL PROTEIN, anticorps KC1, anticorps potassium channel in Arabidopsis thaliana 3, anticorps potassium channel protein, anticorps KAT3
    Sujet
    AGI Code: At4g32650
    KC1 is a regulatory K+ channel subunit that assembles with different inward-rectifying K+ channels to affect their activities. The protein is expressed in extremely low amounts, e.g. a few houndred per cell. Alternative names: AKT4, AtKC1, potassium channel TKC
    Poids moléculaire
    expected: 75.5 kDa, apparent: 75 kDa
    UniProt
    P92960
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