Protein A Protein (HRP)

N° du produit ABIN1044879

Détail du produit

Antigène: Protein A

Origine: Staphylococcus aureus

Hôte: Veuillez nous consulter SVP

Purification/Conjugué: Cette Protein A protéine est marqué à la HRP.

Application: ELISA, Western Blotting (WB)

Specificité: Protein A Peroxidase was prepared from chromatographically pure Protein A. Protein A Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Protein A. No reaction was observed against anti-Protein G.

Information d'application

Indications d'application: Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

Commentaires:

Protein A Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein A Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110). Protein A Peroxidase is stable at 4° C until restored, at which point it is recommended to freeze aliquots at -20° C.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: Protein A

Abstract: Protein A Produits

Synonymes: alpha protein Protein, repB Protein

Sujet: Protein A is a surface protein (approximately 56kDa in size) originally discovered within the cell wall of Staphylococcus aureus. While important for bacterial survival, Protein A has beneficial uses in immunology for its high affinity binding to immunoglobulins (especially the IgG isotype). This high affinity property makes Protein A essential in the large scale purification of antibodies. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).
Synonyms: HRP, peroxidase, Horseradish peroxidase protein A