Annexin V Protein (DY-490)

N° du produit ABIN1047953

Détail du produit

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Type de proteíne: Recombinant

Origine: Souris

Hôte: Veuillez nous consulter SVP

Purification/Conjugué: Cette Annexin V protéine est marqué à la DY-490.

Attributs du produit: Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5

Matériel non inclus: Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml

Information d'application

Indications d'application: 5 l are suitable for one assay

Procédure de l'essai:

Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).

Restrictions: For Research Use only

Stockage

Format: Liquid

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Référence

Vermes, Haanen, Steffens-Nakken, Reutelingsperger: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." dans: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).

Détail du antigène

Antigène: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Autre désignation: Annexin V-Alexa488 (ANXA5 Produits)

ID gène: 11747

UniProt: P48036

Pathways: Apoptose