-
- Antigène Voir toutes Annexin V (ANXA5) Protéines
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- Type de proteíne
- Recombinant
-
Origine
- Souris
- Hôte
- Veuillez nous consulter SVP
- Purification/Conjugué
- Cette Annexin V protéine est marqué à la DY-490.
- Attributs du produit
-
Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
Gene Name: Anxa5 - Matériel non inclus
-
Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
Propidium iodide: 50 g/ml, final concentration 1 g/ml - Top Product
- Discover our top product ANXA5 Protéine
-
-
- Indications d'application
- 5 l are suitable for one assay
- Procédure de l'essai
-
Staining protocol:
1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
3. Incubate for 15min at room temperature in the dark
4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour). - Restrictions
- For Research Use only
-
- Format
- Liquid
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
-
-
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." dans: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
: "
-
A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." dans: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).
-
- Antigène
- Annexin V (ANXA5) (Annexin A5 (ANXA5))
- Autre désignation
- Annexin V-Alexa488 (ANXA5 Produits)
- ID gène
- 11747
- UniProt
- P48036
- Pathways
- Apoptose
-