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Annexin V Protein (DY-490)

ANXA5 Origine: Souris Recombinant
N° du produit ABIN1047953
  • Antigène Voir toutes Annexin V (ANXA5) Protéines
    Annexin V (ANXA5) (Annexin A5 (ANXA5))
    Type de proteíne
    Recombinant
    Origine
    • 17
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    Veuillez nous consulter SVP
    Purification/Conjugué
    Cette Annexin V protéine est marqué à la DY-490.
    Attributs du produit
    Synonyms: Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), Thromboplastin inhibitor, Vascular anticoagulant-alpha (VAC-alpha), Anchorin CII Conjugate: Alexa Fluor 488 Maximum of Absorption at 495 nm, Maximum of Emission at 519 nm
    Gene Name: Anxa5
    Matériel non inclus
    Binding Buffer: 10 mM Hepes pH7.4, 140 mM NaCl, 2.5 mM CaCl2
    Propidium iodide: 50 g/ml, final concentration 1 g/ml
    Top Product
    Discover our top product ANXA5 Protéine
  • Indications d'application
    5 l are suitable for one assay
    Procédure de l'essai

    Staining protocol:
    1. Harvest 2x105 cells per staining and wash cells twice in cold phosphate- buffered-saline (PBS)
    2. Resuspend cells in 100 l binding buffer, add 5l annexin V-Alexa488 to the cells and mix gently.
    For detection of necrosis add additionally appropriate amounts of propidium iodide (final concentration 1g/ml or 1.5M).
    3. Incubate for 15min at room temperature in the dark
    4. Add 400 l binding buffer and analyse the staining in flow cytometry as soon as possible (within an hour).

    Restrictions
    For Research Use only
  • Format
    Liquid
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
  • Vermes, Haanen, Steffens-Nakken, Reutelingsperger: "A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V." dans: Journal of immunological methods, Vol. 184, Issue 1, pp. 39-51, (1995) (PubMed).

  • Antigène
    Annexin V (ANXA5) (Annexin A5 (ANXA5))
    Autre désignation
    Annexin V-Alexa488 (ANXA5 Produits)
    ID gène
    11747
    UniProt
    P48036
    Pathways
    Apoptose
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