Recombinant Human Cysteine Dioxygenase Type 1/CDO1 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Asn200) of Human CDO1 fused with a 6His tag at the N-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 1 mM DTT, 10 % Glycerol, pH 8.0.
Agent conservateur
Dithiothreitol (DTT)
Précaution d'utilisation
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
-80 °C
Stockage commentaire
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
1300002L19Rik Protein, Cdo Protein, D18Ucla3 Protein, cysteine dioxygenase, type I Protein, cysteine dioxygenase 1, cytosolic Protein, cysteine dioxygenase type 1 Protein, NAEGRDRAFT_80458 Protein, Cdo1 Protein, CDO1 Protein
Sub Type
Fusionprotein
Sujet
Cysteine Dioxygenase Type 1 (CDO1) is a mammalian non-heme iron enzyme that belongs to the cysteine dioxygenase family. CDO1 is highly expressed in the liver and placenta, and has a low expression in heart, brain and pancreas. CDO1 can also be detected in hepatoblastoma HepG2 cells. CDO1 catalyzes the conversion of L-cysteine to cysteine sulfinic acid by incorporation of dioxygen. CDO1 is a vital regulator of cellular cysteine concentrations and has an essential role in maintaining the hepatic concentration of intracellular free cysteine within a proper narrow range. CDO1 is able to alter intracellular cysteine levels and glutathione levels. Alternative Names: Cysteine Dioxygenase Type 1, Cysteine Dioxygenase Type I, CDO, CDO-I, CDO1