Recombinant Human Prostatic Acid Phosphatase/ACPP produced by transfected human cells is a secreted protein with sequence (Lys33-Asp386) of Human ACPP fused with a polyhistidine tag at the C-terminus.
Pureté
> 95 % as determined by reducing SDS-PAGE.
Stérilité
0.2 μm filtered
niveau d'endotoxine
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
ACPP
Origine: Humain
Hôte: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5.
Conseil sur la manipulation
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Stock
-80 °C
Stockage commentaire
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
Prostatic Acid Phosphatase (PAP) belongs to the histidine acid phosphatase family. PAP can catalyze the hydrolysis of member of phosphate monoestyers, including phosphorylated protein. PAP can high expression in metastasized prostate cancer, moderately expression level in bone diseases, blood cell disease, and the concentration of PAP is used to monitor and assess the proession of prostate cancer. The optimum PH of PAP is from 4 to 6, its activity can be inhibited by L(+)-tartrate. Alternative Names: Prostatic Acid Phosphatase, PAP, 5'-Nucleotidase, 5'-NT, Ecto-5'-Nucleotidase, Thiamine Monophosphatase, TMPase, ACPP