14-3-3 sigma/SFN Protein (AA 1-248)

N° du produit ABIN1097592

Détail du produit

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Type de proteíne: Recombinant

Attributs du protein: AA 1-248

Origine: Humain

Source: Escherichia coli (E. coli)

Fonction: Recombinant Human Stratifin/SFN

Séquence: MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS

Attributs du produit: Recombinant Human Stratifin is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser248) of Human Stratifin.

Pureté: > 95 % as determined by reducing SDS-PAGE.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Information d'application

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.4.

Agent conservateur: Dithiothreitol (DTT)

Précaution d'utilisation: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Date de péremption: 3 months

Détail du antigène

Antigène: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Autre désignation: Stratifin (SFN Produits)

Synonymes: YWHAS Protein, Er Protein, Mme1 Protein, Ywhas Protein, stratifin Protein, SFN Protein, Sfn Protein

Sujet: Stratifin (SFN) belongs to the 14-3-3 family of proteins that act as important regulators of intracelluar signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least seven isoforms that have been identified in mammals (beta, gamma, epsilon, sigma, zeta, tau and eta). SFN can detected in many tissues, highly expressed in stratified squamous keratinizing epithelium. SFN is indicated as an epithelial cell marker and serves as a tumor suppressor whose expression can be down regulated by methylation. In addition, SFN plays a key role in maintaining the G2 checkpoint in cells and preventing mitotic death.
Alternative Names: 14-3-3 Protein Sigma, Epithelial Cell Marker Protein 1, Stratifin, SFN, HME1

Poids moléculaire: 27.77 kDa

UniProt: P31947

Pathways: Signalisation p53, Myometrial Relaxation and Contraction