ZWINT Protein (AA 1-277) (His tag)
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- Antigène Voir toutes ZWINT Protéines
- ZWINT (ZW10 Interactor (ZWINT))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-277
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette ZWINT protéine est marqué à la His tag.
- Fonction
- Recombinant Human ZW10 Interactor/ZWINT (N-6His)
- Séquence
- MGSSHHHHHH SSGLVPRGSH MEAAETEAEA AALEVLAEVA GILEPVGLQE EAELPAKILV EFVVDSQKKD KLLCSQLQVA DFLQNILAQE DTAKGLDPLA SEDTSRQKAI AAKEQWKELK ATYREHVEAI KIGLTKALTQ MEEAQRKRTQ LREAFEQLQA KKQMAMEKRR AVQNQWQLQQ EKHLQHLAEV SAEVRERKTG TQQELDGVFQ KLGNLKQQAE QERDKLQRYQ TFLQLLYTLQ GKLLFPEAEA EAENLPDDKP QQPTRPQEQS TGDTMGRDPG VSFKAVGLQP AGDVNLP
- Attributs du produit
- Recombinant Human ZW10 Interactor/ZWINT is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro277) of Human ZWINT fused with a His tag at the N-terminus.
- Pureté
- > 95 % as determined by reducing SDS-PAGE.
- Stérilité
- 0.2 μm filtered
- niveau d'endotoxine
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product ZWINT Protéine
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Conseil sur la manipulation
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Stock
- 4 °C/-20 °C/-80 °C
- Stockage commentaire
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Date de péremption
- 3 months
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- Antigène
- ZWINT (ZW10 Interactor (ZWINT))
- Autre désignation
- ZWINT (ZWINT Produits)
- Synonymes
- ZWINT Protein, HZwint-1 Protein, KNTC2AP Protein, ZWINT1 Protein, 2010007E07Rik Protein, 2600001N01Rik Protein, D10Ertd749e Protein, Zwint-1 Protein, Sip30 Protein, ZW10 interacting kinetochore protein Protein, ZW10 interactor Protein, ZWINT Protein, Zwint Protein
- Sub Type
- Fusionprotein
- Sujet
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ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.
Alternative Names: ZW10 Interactor, ZW10-Interacting Protein 1, Zwint-1, ZWINT - Poids moléculaire
- 33.4 kDa
- UniProt
- O95229
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