MDH protein (His tag)
-
- Antigène
- MDH
- Type de proteíne
- Recombinant
-
Origine
- E. coli
-
Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- His tag
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Pureté
- > 95 % by SDS - PAGE
-
-
- Commentaires
-
Synonyms: malate dehydrogenase, ECK3225, JW3205
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
- Stock
- 4 °C
- Stockage commentaire
- Avoid repeated freezing and thawing cycles.
-
- Antigène
- MDH
- Synonymes
- MDH Protein, MMDH Protein, malate dehydrogenase 2 Protein, MDH2 Protein
- Sujet
- Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 34.9kDa (336aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_417703
-