POLR2J3 Protein (His tag)
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- Antigène Voir toutes POLR2J3 Protéines
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- Type de proteíne
- Recombinant
- Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette POLR2J3 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Pureté
- > 95 % by SDS - PAGE
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- Commentaires
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Synonyms: DNA-directed RNA polymerase II subunit RPB11-b2, POLR2J2, RPB11b1, RPB11b2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT
- Stock
- 4 °C
- Stockage commentaire
- Avoid repeated freezing and thawing cycles.
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- Antigène
- POLR2J3 (Polymerase (RNA) II (DNA Directed) Polypeptide J3 (POLR2J3))
- Autre désignation
- POLR2J3 (POLR2J3 Produits)
- Synonymes
- POLR2J2 Protein, RPB11b1 Protein, RPB11b2 Protein, RNA polymerase II subunit J3 Protein, POLR2J3 Protein
- Sujet
- DNA-directed RNA polymerase II subunit RPB11-b2, also known as POLR2J3, belongs to the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exist as three variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is DNA-dependent RNA polymerases that catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2J3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 15.5 kDa (138aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001091084
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