ICT1 Protein (His tag)
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- Antigène Voir toutes ICT1 Protéines
- ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))
- Type de proteíne
- Recombinant
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Origine
- Humain
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette ICT1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Pureté
- > 90 % by SDS - PAGE
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- Commentaires
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Synonyms: Peptidyl-tRNA hydrolase ICT1, mitochondrial, DS-1, DS1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 30% glycerol, 1 MM DTT
- Stock
- 4 °C
- Stockage commentaire
- Avoid repeated freezing and thawing cycles.
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- Antigène
- ICT1 (Immature Colon Carcinoma Transcript 1 (ICT1))
- Autre désignation
- ICT1 (ICT1 Produits)
- Synonymes
- ICT1 Protein, DS-1 Protein, DS1 Protein, 1110001A02Rik Protein, 1110002E03Rik Protein, mitochondrial ribosomal protein L58 Protein, imitochondrial ribosomal protein L58 S homeolog Protein, Immature colon carcinoma transcript 1 Protein, MRPL58 Protein, mrpl58.S Protein, PVX_091510 Protein, mrpl58 Protein, Mrpl58 Protein, ict1 Protein
- Sujet
- Peptidyl-tRNA hydrolase ICT1 acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. It may be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes. Recombinant human ICT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Poids moléculaire
- 22.8 kDa (200aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001536
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