EPO Protéine
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- Antigène Voir toutes EPO Protéines
- EPO (Erythropoietin (EPO))
- Type de proteíne
- Recombinant
- Activité biologique
- Active
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Origine
- Souris
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func)
- Marque
- BD Pharmingen™
- Specificité
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Biological Activity Measured using TF-1 indicator cells
Specific Activity: 0.1 - 1.0 x 10^8 Units/mg (Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation).
ED50: 0.1 - 1.0 ng/ml
Observed linear dose response range: >100 fold - Attributs du produit
- Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, and containing 1 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse EPO is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.
- Purification
- The recombinant mouse EPO is > 95 % pure, as determined by SDS-PAGE and an absorbance assay based on Beers-Lambert law.
- Pureté
- ≥ 95 %
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- The endotoxin level is ≤ 0.1 ng per µg of mouse EPO, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product EPO Protéine
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Commentaires
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BD Pharmingen™ Mouse Erythropoietin Recombinant Protein - Reactivity Ms
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1 mg/mL
- Buffer
- Recombinant mouse EPO is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, and containing 1 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.
- Agent conservateur
- Azide free
- Conseil sur la manipulation
- Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
- Stock
- -80 °C
- Stockage commentaire
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
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Nucleotide sequence of rat erythropoietin." dans: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).
: "Erythropoietin." dans: Blood, Vol. 77, Issue 3, pp. 419-34, (1991) (PubMed).
: "Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin." dans: Journal of cellular physiology, Vol. 140, Issue 2, pp. 323-34, (1989) (PubMed).
: "Cloning, sequencing, and evolutionary analysis of the mouse erythropoietin gene." dans: Molecular and cellular biology, Vol. 6, Issue 3, pp. 842-8, (1986) (PubMed).
: "Murine erythropoietin gene: cloning, expression, and human gene homology." dans: Molecular and cellular biology, Vol. 6, Issue 3, pp. 849-58, (1986) (PubMed).
: "Role of the kidney in erythropoiesis." dans: Nature, Vol. 179, Issue 4560, pp. 633-4, (1957) (PubMed).
: "
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Nucleotide sequence of rat erythropoietin." dans: Biochimica et biophysica acta, Vol. 1171, Issue 1, pp. 99-102, (1992) (PubMed).
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- Antigène
- EPO (Erythropoietin (EPO))
- Autre désignation
- EPO (EPO Produits)
- Synonymes
- EPO Protein, EP Protein, MVCD2 Protein, erythropoietin Protein, erythropoietin S homeolog Protein, erythropoietin a Protein, EPO Protein, epo Protein, epo.S Protein, Epo Protein, epoa Protein
- Classe de substances
- Hormone
- Sujet
- Mouse erythropoietin (EPO) is a 30 kD heavily glycosylated protein containing 166 amino acids. The carbohydrate residues compose approximately 30 % of the molecule by weight.3 It shares 80 % and 95 % homology with human and rat EPO, respectively. EPO functions as the survival and proliferation factors of late erythroid progenitor cells (CFU-E). In adult mammals, EPO is synthesized almost exclusively in the kidneys.
- Pathways
- Signalistation JAK/STAT, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
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